Human Rantes ELISA Kit
KOA0293
1 Kit
ELISA
Human
15.6 pg/ml - 1000 pg/ml
Shipping info:
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Product Details
Human Rantes AccuSignal ELISA Kit - KOA0293
Beta chemokine RANTES, Beta chemokine RANTES precursor, C C motif chemokine 5, CCL5_HUMAN, Chemokine ligand 5, Chemokine CC Motif Ligand 5, EoCP, Eosinophil chemotactic cytokine, RANTES, Regulated upon activation normally T expressed and presumably secreted, SCYA5, SIS delta, SIS-delta, SISd, Small inducible cytokine A5, Small inducible cytokine subfamily A member 5, T cell specific protein p288, T cell specific protein RANTES, T-cell-specific protein RANTES, TCP 228, TCP228
ELISA Kit
15.6 pg/ml - 1000 pg/ml
Target Details
CCL5 - View All CCL5 Products
Human
Expression system for standard: E.coli; Immunogen sequence: S24-S91
Natural and recombinant human Rantes. There is no detectable cross-reactivity with other relevant proteins.
D0EI67 - UniProtKB
NP_002976.2 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human Rantes standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human Rantes standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Rantes(regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
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