Datasheet is currently unavailable. Try again or CONTACT US
PDCD1 Antibody
Rabbit Polyclonal
600-401-A72
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IF
Human, Mouse, Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-PDCD1 (RABBIT) Antibody - 600-401-A72
PD1 antibody, PDCD 1 antibody, CD279 antibody
Rabbit
Polyclonal
IgG
Target Details
PDCD1 - View All PDCD1 Products
Human, Mouse, Rat
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human PDCD1 protein.
This affinity purified antibody is directed against human PDCD1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with PDCD1 from human, mouse and rat sources. Reactivity with PDCD1 from other sources has not been determined.
Application Details
ELISA, IF, IHC, WB
This Anti-PDCD1 antibody has been tested for use in ELISA, immunofluorescence, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~47 kDa in size corresponding to PDCD1 by western blotting in the appropriate cell lysate or extract. THP-1 cell lysate can be used as positive control.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Cell-mediated immune responses are initiated by T lymphocytes th Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PDCD-1 that mediate inhibitory signals from the APC. PDCD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PDCD1 inhibit the activation of the immune response in the absence of “danger signals” such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PDCD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PDCD1 often migrates at higher molecular weight in SDS-PAGE.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
