Collagen Type II Antibody
Rabbit Polyclonal
27 References
600-401-104S
600-401-104-0.1
600-401-104-0.5
25 µL
100 µg
500 µg
Liquid (sterile filtered)
Liquid (sterile filtered)
Liquid (sterile filtered)
WB, IHC, IF, Dot Blot, Multiplex
Human, Bovine
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Collagen Type II (RABBIT) Antibody - 600-401-104-0.1
rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody
Rabbit
Polyclonal
IgG
Target Details
COL2A1 - View All COL2A1 Products
Human, Bovine
Native Protein
Collagen Type II from human knee cartilage and bovine nasal cartilage.
Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
Application Details
Dot Blot, IHC
IF, WB, Multiplex
- View References
Anti-Collagen Type II has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.
Formulation
1.15 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.
Zappa F et al. (2024). Role of trafficking protein particle complex 2 in medaka development. Traffic.
Applications
IF, Confocal Microscopy; WB, IB, PCA
Unlu G, Qi X, Gamazon ER, et al. (2020). Phenome-based approach identifies RIC1-linked Mendelian syndrome through zebrafish models, biobank associations and clinical studies. Nat Med.
Applications
IF, Confocal Microscopy
Lee M, Bae K, Levinson C, Zenobi-Wong M. (2020). Nanocomposite bioink exploits dynamic covalent bonds between nanoparticles and polysaccharides for precision bioprinting. Biofabrication.
Applications
IHC, ICC, Histology
Taniguchi D, Matsumoto K, Machino R, et al. (2020). Human lung microvascular endothelial cells as potential alternatives to human umbilical vein endothelial cells in bio-3D-printed trachea-like structures. Tissue Cell.
Applications
IHC, ICC, Histology
Li F et al. (2020). Microencapsulation improves chondrogenesis in vitro and cartilaginous matrix stability in vivo compared to bulk encapsulation. Biomed Mater. Sci.
Applications
IHC, ICC, Histology
Levinson C et al. (2020). Is there a scientific rationale for the refixation of delaminated chondral flaps in femoroacetabular impingement? a laboratory study. Clin Orthop Relat Res.
Applications
IHC, ICC, Histology
Ozturk E et al. (2020). Tyrosinase-crosslinked, tissue adhesive and biomimetic alginate sulfate hydrogels for cartilage repair. Biomed Mater.
Applications
Undefined
Forrester A, De Leonibus C, Grumati P, et al. (2019). A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex. EMBO J.
Applications
IF, Confocal Microscopy
Cavalli E, Levinson C, Hertl M, et al. (2019). Characterization of polydactyly chondrocytes and their use in cartilage engineering. Sci Rep.
Applications
IHC, ICC, Histology
Levinson C, Cavalli E, Sindi DM, et al. (2019). Chondrocytes From Device-Minced Articular Cartilage Show Potent Outgrowth Into Fibrin and Collagen Hydrogels. Orthop J Sports Med.
Applications
IHC, ICC, Histology
Levinson C, Lee M, Applegate LA, Zenobi-Wong M. (2019). An injectable heparin-conjugated hyaluronan scaffold for local delivery of transforming growth factor β1 promotes successful chondrogenesis. Acta Biomater.
Applications
IHC, ICC, Histology
Schuelke J, Meyers N, Reitmaier S, Klose S, Ignatius A, Claes L. (2018). Intramembranous bone formation after callus distraction is augmented by increasing axial compressive strain. PLoS One.
Applications
IHC, ICC, Histology
EmmaCavalli et al. (2018). A comparative study of cartilage engineered constructs in immunocompromised, humanized and immunocompetent mice. Journal of Immunology and Regenerative Medicine
Applications
IHC, ICC, Histology
Taniguchi D et al. (2018). Scaffold-free trachea regeneration by tissue engineering with bio-3D printing. Inetact Cardiovasc Thorac Surg.
Applications
IHC, ICC, Histology
Formica FA et al. (2018). Cell-Instructive Alginate Hydrogels Targeting RhoA. Bioconjug Chem.
Applications
IHC, ICC, Histology
He et al. (2017). Repair of osteochondral defects with in vitro engineered cartilage based on autologous bone marrow stromal cells in a swine model. Scientific Reports
Applications
IHC, ICC, Histology
Morgese G et al. (2017). Nanoassemblies of tissue-reactive, polyoxazoline graft-copolymers restore the lubrication properties of degraded cartilage. ACS Nano.
Applications
IHC, ICC, Histology
Formica FA, Öztürk E, Hess SC, et al. (2016). A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular Matrix. Adv Healthc Mater.
Applications
IHC, ICC, Histology
Nicolas Broguiere et al. (2016). Factor XIII Cross-Linked Hyaluronan Hydrogels for Cartilage Tissue Engineering. ACS Biomater. Sci. Eng.
Applications
IHC, ICC, Histology
Broguiere N et al. (2016). Factor XIII cross-linked hyaluronan hydrogels for cartilage tissue engineering. ACS Biomater Sci Eng.
Applications
IHC, ICC, Histology
Martins EA, Michelacci YM, Baccarin RY, Cogliati B, Silva LC. (2014). Evaluation of chitosan-GP hydrogel biocompatibility in osteochondral defects: an experimental approach. BMC Vet Res.
Applications
IF, Confocal Microscopy
Liedert, A et al. (2014). Osteoblast-specific Krm2 overexpression and Lrp5 deficiency have different effects on fracture healing in mice. PloS One
Applications
IHC, ICC, Histology
Rojewski MT, Fekete N, Baila S, et al. (2013). GMP-compliant isolation and expansion of bone marrow-derived MSCs in the closed, automated device quantum cell expansion system. Cell Transplant.
Applications
IHC, ICC, Histology
Lawson KA, Teteak CJ, Zou J, et al. (2013). Mesenchyme-specific knockout of ESET histone methyltransferase causes ectopic hypertrophy and terminal differentiation of articular chondrocytes. J Biol Chem.
Applications
IF, Confocal Microscopy
Gomer RH, Pilling D, Kauvar LM, et al. (2009). A serum amyloid P-binding hydrogel speeds healing of partial thickness wounds in pigs. Wound Repair Regen.
Applications
IHC, ICC, Histology
Pilling D, Roife D, Wang M, et al. (2007). Reduction of bleomycin-induced pulmonary fibrosis by serum amyloid P. J Immunol.
Applications
IHC, ICC, Histology
Kondo S, Shukunami C, Morioka Y, et al. (2007). Dual effects of the membrane-anchored MMP regulator RECK on chondrogenic differentiation of ATDC5 cells. J Cell Sci.
Applications
IF, Confocal Microscopy; Multiplex Assay
What were the immunogens for the collagen antibodies?
The immunogens for the collagen antibodies are the respective purified solubilized native collagen proteins (tropocollagens).
In what format are the collagen antibodies provided?
The collagen antibodies are provided in liquid format at a concentration of around 1 mg/mL (Absorbance 280 nm).
In what buffer are the collagen antibodies provided?
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide.
Are the antibodies specific to the reduced or non-reduced (native) proteins?
The collagen antibodies are specific to the native 3D epitopes found on the collagen native collagen protein. The collagen antibodies may have no reactivity to reduced collagen.
How was the reactivity of the antibodies tested? Was cross-reactivity to other collagens tested?
Each antibody was tested by immunoblot against each type of collagen (Type I – VI). Due to the close association of collagen proteins, cross-reactivity to other collagens may be observed. The optimal experimental conditions should be determined to minimize cross-reactivity in your application.
What are the recommended storage conditions for collagen antibodies?
The recommended storage temperature for collagen antibodies is -20°C. Please be sure to add glycerol 1:1 before freezing. We recommend making aliquots for best stability. The antibodies are stable for several weeks at 4°C.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
