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Datasheet

Eaat2 Antibody

Goat Polyclonal

600-101-MN3S 600-101-MN3
25 µL 100 µg
Liquid (sterile filtered) Liquid (sterile filtered)
WB, IF
Mouse, Rat
Goat
$125.00 /Per Item
$475.00 /Per Item
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Product Details

Anti-Eaat2 (GOAT) Antibody - 600-101-MN3
Goat Anti-Eaat2 Antibody, Goat Anti-Excitotoxic Amino Acid Transporter Antibody, Solute Carrier Family 1 Member 2, Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter) Member 2, Sodium-Dependent Glutamate/Aspartate Transporter 2, Toxcitatory Amino Acid Transporter, Glutamate/Aspartate Transporter II, EAAT2, Excitotoxic Amino Acid Transporter, EIEE41, GLT-1, HBGT, GLT1
Goat
Polyclonal
IgG

Target Details

Slc1a2  - View All Slc1a2 Products
Mouse, Rat
Conjugated Peptide
Anti-Eaat2 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminal region of Rat Eaat2 surrounding aa1-25 conjugated to Keyhole Limpet Hemocyanin (KLH).
This affinity purified antibody is directed against rat Eaat2.  This product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, and rat.
P31596 - UniProtKB
NP_001289018.1 - NCBI Protein
29482 - Gene ID

Application Details

IF, WB
Anti-Eaat2 Antibody has been tested in WB and IF.  Expect a band at ~62kDa in western blot using appropriate tissues and lysates.  Positive control used: rat and mouse brain lysates in western blot and Rat Pups Brain heterogenous cells in immunofluorescence.

Formulation

1.05 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
SLC1A2 (Solute Carrier Family 1 Member 2), glutamate transporters, also known as excitatory amino acid transporters (EAATs), are sodium- and potassium- dependent members of the solute carrier family 6 (SLC1), widely distributed throughout the brain. These sodium-dependent, high-affinity amino acid transporter mediates the uptake of L-glutamate, L-aspartate, and D-aspartate functioning as a symporter that transports one amino acid molecule together with two or three Na (+) ions and one proton, in parallel with the counter-transport of one K (+) ion. It mediates the Cl (-) flux that is not coupled to amino acid transport avoiding the accumulation of negative charges due to aspartate and Na (+) symport. Eaat2 is essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate. Improper regulation of this gene is thought to be associated with several neurological disorders including Epileptic Encephalopathy, Early Infantile, 41 and Undetermined Early-Onset Epileptic Encephalopathy. Anti-Eaat2 Antibody is useful for researchers interested in transmembrane transporter activity, neuroscience research, and cell metabolism.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.