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ASPP2 Antibody
Rabbit Polyclonal
1 References
200-401-A19
100 µg
Liquid (sterile filtered)
WB, ELISA
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-ASPP2 (RABBIT) Antibody - 200-401-A19
rabbit anti-ASPP2 antibody, ASPP-2, ASPP 2, Apoptosis stimulating of p53 protein 2 antibody, Bbp antibody, Bcl2 binding protein antibody, NY REN 51 antigen antibody, p53 binding protein 2 antibody, Tumor suppressor p53-binding protein 2, TP53BP2
Rabbit
Polyclonal
IgG
Target Details
TP53BP2 - View All TP53BP2 Products
Human
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP2.
This Protein A purified antibody is directed against human ASPP2. The product was purified from monospecific antiserum by Protein A affinity chromatography. Minimal reactivity occurs against ASPP1. A BLAST analysis was used to suggest cross-reactivity with ASPP2 from macaque and dog sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with ASPP2 from mouse and rat sources based on an 80% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Application Details
ELISA, WB
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kDa in size corresponding to ASPP2 by western blotting in the appropriate cell lysate or extract.
Formulation
2.4 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.
Sharafutdinov I et al, (2024). Cortactin-dependent control of Par1b-regulated epithelial cell polarity in Helicobacter infection. Cell Insight.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.