SDS-PAGE of Mouse IgM Kappa (κ) isotype control (p/n 010-001-339)

Datasheet

Mouse IgM Kappa isotype Control

Name: Mouse IgM Kappa isotype Control
Brand: Rockland Immunochemicals
Price: 210.0 USD
Availability: InStock
Rating: 5 (1 reviews)

1 References

Item No. 010-001-339

Size: 1 mg

Physical State: Liquid (sterile filtered)

Applications: ELISA, SDS-PAGE

Origin: Mouse

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Specifications for Mouse IgM Kappa isotype Control

Product Details

Description: Mouse IgM Kappa (k) Isotype Control - 010-001-339

Synonyms: Mouse immunoglobulin M kappa, Mouse IgM isotype control, mouse IgM kappa

Species of Origin: Mouse

Format: IgM

Type: Native Protein

Target Details

Purity/Specificity: Mouse IgM Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, Ig3, IgA. Light chain composition has been confirmed by SDS-PAGE.

Application Details

Tested Applications: SDS-PAGE

Suggested Applications: ELISA - View References

Application Note: Mouse IgM kappa isotype control has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Formulation

Concentration: 1.0mg/mL by UV absorbance at 280 nm

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Preservative: 0.1% (w/v) Sodium Azide

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. Mouse IgM Kappa (κ) isotype control is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.

References (1)

Sawada N et al. (2020). Circulating oxidized LDL, increased in patients with acute myocardial infarction, is accompanied by heavily modified HDL. J Lipid Res.

Applications

EIA, ELISA; Comparative quantitation of DNA by UV and LC-MS, MS, UPLC-MSMS

PubMed

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.