Collagen Type IV Antibody
Rabbit Polyclonal
45 References
600-401-106S
600-401-106-0.1
600-401-106-0.5
25 µL
100 µg
500 µg
Liquid (sterile filtered)
Liquid (sterile filtered)
Liquid (sterile filtered)
WB, IHC, IF, Dot Blot, EM, Multiplex
Human, Bovine
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Collagen Type IV (RABBIT) Antibody - 600-401-106-0.1
rabbit anti-Collagen Type IV antibody, Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, Collagen alpha-1 (IV) chain, COL4A1
Rabbit
Polyclonal
IgG
Target Details
COL4A1-COL4A6 - View All COL4A1-COL4A6 Products
Human, Bovine
Native Protein
Collagen Type IV from human and bovine placenta
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Application Details
Dot Blot, IHC
EM, IF, WB, Multiplex
- View References
Anti-Collagen Type IV has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
Formulation
1.18 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
Chiusa M et al. (2024). Cytoplasmic retention of the DNA/RNA-binding protein FUS ameliorates organ fibrosis in mice. J Clin Invest.
Applications
IF, Confocal Microscopy; WB, IB, PCA
Yu Z et al. (2022). The latent dedifferentiation capacity of newt limb muscles is unleashed by a combination of metamorphosis and body growth. Scientific Reports
Applications
IHC, ICC, Histology
Ida, Y et al. (2022). ROCK 1 and 2 affect the spatial architecture of 3D spheroids derived from human corneal stromal fibroblasts in different manners. Scientific Reports
Applications
IF, Confocal Microscopy
Suzuki, S et al. (2022). Comparison of the Drug-Induced Efficacies between Omidenepag Isopropyl, an EP2 Agonist and PGF2α toward TGF-β2-Modulated Human Trabecular Meshwork (HTM) Cells. Journal of Clinical Medicine
Applications
IF, Confocal Microscopy
Sasaki K et al. (2022). Macrophage interferon regulatory factor 4 deletion ameliorates aristolochic acid nephropathy via reduced migration and increased apoptosis. JCI Insight
Applications
IHC, ICC, Histology
Hu C et al. (2022). Multiomic identification of factors associated with progression to cystic kidney disease in mice with nephron Ift88 disruption. Am J Physiol Renal Physiol.
Applications
WB, IB, PCA
Ida Y et al. (2022). Addition of ROCK Inhibitors Alleviates Prostaglandin-Induced Inhibition of Adipogenesis in 3T3L-1 Spheroids. Bioengineering (Basel).
Applications
IF, Confocal Microscopy
Kumar AA et al. (2022). Vascular Collagen Type-IV in Hypertension and Cerebral Small Vessel Disease. Stroke.
Applications
IHC, ICC, Histology; Immuno-EM
Mollo N et al. (2022). Overexpression of the Hsa21 Transcription Factor RUNX1 Modulates the Extracellular Matrix in Trisomy 21 Cells. Front Genet.
Applications
WB, IB, PCA
Tsugeno Y et al. (2022). All Trans-Retinoic Acids Facilitate the Remodeling of 2D and 3D Cultured Human Conjunctival Fibroblasts. Bioengineering (Basel).
Applications
IF, Confocal Microscopy
Sasaki K et al. (2021). Deletion of Myeloid Interferon Regulatory Factor 4 (Irf4) in Mouse Model Protects against Kidney Fibrosis after Ischemic Injury by Decreased Macrophage Recruitment and Activation. J Am Soc Nephrol.
Applications
Undefined
Nandadasa S et al. (2021). The versican-hyaluronan complex provides an essential extracellular matrix niche for Flk1+ precursors in vasculogenesis and primitive hematopoiesis. Matrix Biol.
Applications
IF, Confocal Microscopy
Ida Y. et al. (2020). Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell adipogenesis affects spatial formation of extra-cellular matrix. Sci Rep
Applications
IF, Confocal Microscopy
Liu S. et al. (2020). Inhibition of RNA-binding protein HuR reduces glomerulosclerosis in experimental nephritis. Clin Sci (Lond).
Applications
IF, Confocal Microscopy
Cai C, Zhao C, Kilari S, et al. (2020). Effect of sex differences in treatment response to angioplasty in a murine arteriovenous fistula model. Am J Physiol Renal Physiol.
Applications
IHC, ICC, Histology
Keller F et al. (2020). A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro. Cells.
Applications
IF, Confocal Microscopy
Li Y et al. (2020). Podocyte EGFR Inhibits Autophagy Through Upregulation of Rubicon in Type 2 Diabetic Nephropathy. Diabetes
Applications
IHC, ICC, Histology
Kumar G et al. (2020). Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids. Communications Biology
Applications
IF, Confocal Microscopy
Hikage F, Atkins S, Kahana A, Smith TJ, Chun TH. (2019). HIF2A-LOX Pathway Promotes Fibrotic Tissue Remodeling in Thyroid-Associated Orbitopathy. Endocrinology.
Applications
IF, Confocal Microscopy
Chen H, Huang Y, Zhu X, et al. (2019). Histone demethylase UTX is a therapeutic target for diabetic kidney disease. J Physiol.
Applications
IHC, ICC, Histology
Sumeda Nandadasa et al. (2019). The versican-hyaluronan complex provides an essential extracellular matrix niche for Flk1+ precursors in vasculogenesis and primitive hematopoiesis. bioRxiv
Applications
IF, Confocal Microscopy
Yue CH et al. (2019). Myeloid Zinc Finger 1 (MZF1) Maintains the Mesenchymal Phenotype by Down-regulating IGF1R/p38 MAPK/ERα Signaling Pathway in High-level MZF1-expressing TNBC cells. Anticancer Res.
Applications
IF, Confocal Microscopy
Klicks, J et al. (2019). A novel spheroid-based co-culture model mimics loss of keratinocyte differentiation, melanoma cell invasion, and drug-induced selection of ABCB5-expressing cells. Bmc Cancer
Applications
IF, Confocal Microscopy
Li et al. (2018). Fatty acid receptor modulator PBI-4050 inhibits kidney fibrosis and improves glycemic control. JCI Insight
Applications
IHC, ICC, Histology
Akama T, Chun TH. (2018). Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells. J Biol Chem.
Applications
IF, Confocal Microscopy
Hwang et al. (2017). In Situ Imaging of Tissue Remodeling with Collagen Hybridizing Peptides. ACS Nano
Applications
CHP Staining
Wang X, Yao B, Wang Y, et al. (2017). Macrophage Cyclooxygenase-2 Protects Against Development of Diabetic Nephropathy. Diabetes. 2017;66(2):494-504. doi:10.2337/db16-0773 Diabetes.
Applications
IHC, ICC, Histology
Ma S, Sun R, Jiang B, et al. (2017). L2hgdh Deficiency Accumulates l-2-Hydroxyglutarate with Progressive Leukoencephalopathy and Neurodegeneration. Mol Cell Biol.
Applications
Undefined
Tanaka et al. (2016). A developmentally regulated switch from stem cells to dedifferentiation for limb muscle regeneration in newts. Nature Communications
Applications
IHC, ICC, Histology; Multiplex Assay
Mi et al. (2016). Tenascin-C Is Associated with Cored Amyloid-β Plaques in Alzheimer Disease and Pathology Burdened Cognitively Normal Elderly. Journal of Neuropathology & Experimental Neurology
Applications
IHC, ICC, Histology
Dubail et al. (2016). Impaired ADAMTS9 secretion: A potential mechanism for eye defects in Peters Plus Syndrome. Scientific Reports
Applications
IHC, ICC, Histology
Sharmin S, Taguchi A, Kaku Y, et al. (2016). Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation. J Am Soc Nephrol.
Applications
IHC, ICC, Histology
Ma S, Jiang B, Deng W, et al. (2015). D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth. Oncotarget.
Applications
WB, IB, PCA
Fetting JL, Guay JA, Karolak MJ, et al. (2014). FOXD1 promotes nephron progenitor differentiation by repressing decorin in the embryonic kidney. Development.
Applications
IHC, ICC, Histology
Chun TH, Inoue M. (2014). 3-D adipocyte differentiation and peri-adipocyte collagen turnover. Methods Enzymol.
Applications
IHC, ICC, Histology; IF, Confocal Microscopy
Chatterjee A, Villarreal G Jr, Oh DJ, Kang MH, Rhee DJ. (2014). AMP-activated protein kinase regulates intraocular pressure, extracellular matrix, and cytoskeleton in trabecular meshwork. Invest Ophthalmol Vis Sci.
Applications
WB, IB, PCA
Murray M et al. (2013). Progressive amnestic dementia, hippocampal sclerosis, and mutation in C9ORF72. Acta Neuropathologica
Applications
IHC, ICC, Histology
Seifert AW, Monaghan JR, Voss SR, Maden M. (2012). Skin regeneration in adult axolotls: a blueprint for scar-free healing in vertebrates. PLoS One.
Applications
IF, Confocal Microscopy
Sasaki M, Knobbe CB, Itsumi M, et al. (2012). D-2-hydroxyglutarate produced by mutant IDH1 perturbs collagen maturation and basement membrane function. Genes Dev.
Applications
WB, IB, PCA; IHC, ICC, Histology
Seifert AW, Kiama SG, Seifert MG, Goheen JR, Palmer TM, Maden M. (2012). Skin shedding and tissue regeneration in African spiny mice (Acomys). Nature.
Applications
IHC, ICC, Histology
Wiessner C, Wiederhold KH, Tissot AC, et al. (2011). The second-generation active Aβ immunotherapy CAD106 reduces amyloid accumulation in APP transgenic mice while minimizing potential side effects. J Neurosci.
Applications
IHC, ICC, Histology
Villarreal G Jr, Oh DJ, Kang MH, Rhee DJ. (2011). Coordinated regulation of extracellular matrix synthesis by the microRNA-29 family in the trabecular meshwork. Invest Ophthalmol Vis Sci.
Applications
WB, IB, PCA
Pinheiro SVB, Ferreira AJ, Kitten GT, et al. (2009). Genetic deletion of the angiotensin-(1-7) receptor Mas leads to glomerular hyperfiltration and microalbuminuria. Kidney Int.
Applications
IHC, ICC, Histology
Morrison, JI. et al (2006). Salamander limb regeneration involves the activation of a multipotent skeletal muscle satellite cell population. The Journal of Cell Biology
Applications
IF, Confocal Microscopy
Kirkland SC, Henderson K. (2001). Collagen IV synthesis is restricted to the enteroendocrine pathway during multilineage differentiation of human colorectal epithelial stem cells. J Cell Sci.
Applications
IF, Confocal Microscopy; IHC, ICC, Histology
What were the immunogens for the collagen antibodies?
The immunogens for the collagen antibodies are the respective purified solubilized native collagen proteins (tropocollagens).
In what format are the collagen antibodies provided?
The collagen antibodies are provided in liquid format at a concentration of around 1 mg/mL (Absorbance 280 nm).
In what buffer are the collagen antibodies provided?
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide.
Are the antibodies specific to the reduced or non-reduced (native) proteins?
The collagen antibodies are specific to the native 3D epitopes found on the collagen native collagen protein. The collagen antibodies may have no reactivity to reduced collagen.
How was the reactivity of the antibodies tested? Was cross-reactivity to other collagens tested?
Each antibody was tested by immunoblot against each type of collagen (Type I – VI). Due to the close association of collagen proteins, cross-reactivity to other collagens may be observed. The optimal experimental conditions should be determined to minimize cross-reactivity in your application.
What are the recommended storage conditions for collagen antibodies?
The recommended storage temperature for collagen antibodies is -20°C. Please be sure to add glycerol 1:1 before freezing. We recommend making aliquots for best stability. The antibodies are stable for several weeks at 4°C.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
