Urm1 Antibody
Anti-Urm1 Antibody - Western Blot
Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs).
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Datasheet

Urm1 Antibody

Rabbit Polyclonal

200-401-443
500 µg
Lyophilized
WB
Yeast
Rabbit
$525.00 /Per Item
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Product Details

Anti-Urm1 (RABBIT) Antibody - 200-401-443
rabbit anti-Urm1 Antibody, C9orf74 antibody, Chromosome 9 open reading frame 74 antibody, MGC2668 antibody, RP11 339B21.4 antibody, Ubiquitin Related Modifier 1 antibody, Ubiquitin related modifier 1 homolog antibody, Urm 1 antibody
Rabbit
Polyclonal
IgG

Target Details

URM1  - View All URM1 Products
Yeast
Recombinant Protein
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Urm1 protein.
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
P40554 - UniProtKB
P40554.1 - NCBI Protein
854809 - Gene ID

Application Details

WB
This purified polyclonal antibody reacts with yeast Urm1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates).  Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 12 kDa band corresponding to yeast Urm1 is detected.  Most yeast cell lysates can be used as a positive control without induction or stimulation. 

Formulation

5.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
100 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Urm1 is a newly identified ubiquitin related modifier.  Urm 1 is a 99-amino acid protein terminated with glycine-glycine.  Target proteins are conjugated to Urm1 via its C-terminal glycine. Initially Urm1 forms a thioester with a novel E1-like protein, Uba4.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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