HER2 Signaling Antibodies

Human epidermal growth factor receptor 2 (HER2) is a member of the epidermal growth factor receptor (HER/EGFR/ERBB) family. It is also called ERBB2, CD340, or proto-oncogene Neu. The protein is a receptor tyrosine kinase located on the plasma membrane of cells. Activation of the tyrosine kinase promotes cell proliferation and suppresses apoptosis. HER2 can dimerize with any other member of the ErbB family, which results in auto-phosphorylation of tyrosine residues and activation of signaling pathways. The signal is then transferred via PI3K/Akt, RAS/MEK/MAPK, and STAT kinase that can be detected using our phospho-specific antibodies to accurately determine activation of the pathway.

Figure: Her2 signaling pathway (Created with BioRender.com)

HER2 is over-expressed in a significant proportion of cancer patients resulting in an increased disease recurrence and poor prognosis. Therefore, an early diagnosis of the HER2 status in the tumors is necessary to decide the appropriate treatment. Furthermore, in breast cancer, HER2 is over-expressed in a variety of different tumors which makes it a target for immunotherapy of certain patients. The antibody Trastuzumab (Herceptin) which recognizes HER2 was approved for the treatment of HER2-positive breast cancer patients in 1998, as the original approval was for the treatment of HER2-positive metastatic breast tumors. Later studies have shown that Herceptin is also beneficial for early-stage HER2-positive tumor patients and is approved as an adjuvant treatment. Additionally, the antibody is now approved for other HER2-positive tumors, such as metastatic gastric adenocarcinoma.

Rockland's antibodies exhibit high specificity and selectivity for the detection of HER2, HER2 ligands, and key proteins of the affected pathways and are routinely used for reproducible detection by Western blotting, immunohistochemistry, immunofluorescence, and ELISA. Accurate detection and quantification of HER2 in complex matrices, including cell culture supernatant, serum or plasma, and tissue homogenates, can be achieved using our Human ErbB-2 AccuSignal ELISA Kit.

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Her2 Signaling Pathway Antibodies

Her2 Signaling Pathway Proteins