DyLight™ conjugated antibodies are high-performance fluorescent conjugates designed for use in various primary and secondary antibody assays, including fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening, multiplex assays, and other array platforms. These conjugates are highly functional with bright emission spectra that align with the output wavelengths of common fluorescence instrumentation. The introduction of DyLight™ conjugates has revolutionized the field of fluorescence-based assays, offering researchers tools with high fluorescence intensity, photostability, and compatibility with standard laboratory equipment, ensuring accurate and reliable results across diverse applications.
DyLight™ Fluorochromes
| Emission | Fluorochrome | Abs (nm) | Em (nm) | MW (daltons) | Spectrally similar |
| Blue | DyLight™ 405 | 420 | 400 | 793 | Alexa Fluor 350 |
| Green | DyLight™ 488 | 493 | 518 | 1,011 | Alexa Fluor 488, Fluorescein |
| Yellow | DyLight™ 549 | 550 | 568 | 982 | ATTO 532, Rhodamine, R-Phycoerythrin, Cy3™ |
| Red | DyLight™ 649 | 649 | 674 | 1,008 | ATTO 655, Allophycocyanin, Cy5™ |
| Near-infrared | DyLight™ 680 | 682 | 715 | 950 | IRDye®700, Alexa Fluor 680, Cy5.5™ |
| Infrared | DyLight™ 800 | 770 | 794 | 1,050 | IRDye®800, Alexa Fluor 790, Cy7™ |
High Fluorescence Intensity & Photostability
- High-intensity emission provides outstanding sensitivity and requires less conjugate
- Negligible bleed-through between fluorophore channels enables multicolor detection
- Exceptional resistance to photobleaching
- Buffers are completely stable at pH 4-9
- DyLight™ is compatible with filter and laser settings on popular fluorescence instrumentation
DyLight™ 405 Conjugates
603-146-126
3 References
Applications
IF, Multiplex
610-146-002
3 References
Applications
IHC, IF, Multiplex
DyLight™ 488 Conjugates
600-141-215
23 References
Applications
WB, IHC, IF, FC, Dot Blot, IP, Multiplex
611-741-127
9 References
Applications
WB, IHC, IF, Dot Blot, Multiplex
605-741-125
7 References
Applications
IF, Dot Blot, Multiplex
610-741-124
6 References
Applications
WB, IHC, IF, Dot Blot, Multiplex
Dylight™ 549 Conjugates
611-142-002
8 References
Applications
WB, IHC, IF, Dot Blot, Multiplex
611-742-127
7 References
Applications
WB, IHC, IF, Dot Blot, Multiplex
600-442-378
6 References
Applications
IHC, IF, EM, Multiplex
610-742-124
6 References
Applications
WB, IHC, IF, Multiplex
DyLight™ 649 Conjugates
611-743-127
8 References
Applications
IHC, IF, Dot Blot, Multiplex
200-343-382
5 References
Applications
WB, ELISA, Dot Blot, Biochemical Assay, Microarray
610-443-040
6 References
Applications
Microarray
612-143-002
3 References
Applications
WB, IF, Multiplex
DyLight™ 680 Conjugates
611-144-002
35 References
Applications
WB, IF, Dot Blot, IP
610-144-002
30 References
Applications
WB, IHC, IF, Dot Blot, IP
611-144-122
9 References
Applications
WB, Dot Blot, FISH, Multiplex
611-744-127
8 References
Applications
WB, Dot Blot
DyLight™ 800 Conjugates
611-145-002
67 References
Applications
WB, ELISA, IHC, Dot Blot, EMSA, IP
610-145-002
57 References
Applications
WB, IHC, IF, Dot Blot, IP
610-145-121
29 References
Applications
WB, IF, Dot Blot
611-145-122
22 References
Applications
WB, Dot Blot, Other