Custom Polyclonal Antibodies

Our Custom Polyclonal Antibody Production Process

Each project is performed in distinct phases—and you decide if and when to initiate each phase.

Phase 1: Antigen Preparation

We can use a range of macromolecules as antigens, as long as they can be used under BSL-2 conditions, and can design and produce custom peptides and recombinant proteins.

• Protein, recombinant or purified
• Synthetic peptide
Modified peptide
• Haptens
• Whole cell lysate
• Nucleic acid
• Lipids
• Carbohydrate complexes
• And more (i.e. viruses, bacteria, toxins, etc.)

Phase 2: Polyclonal Antibody Development

During polyclonal antibody development, we immunize based on the schedule you request and can recommend an adjuvant to achieve your project goals. We provide a pre-bleed serum (5 mL) for screening in your labs and can also use our experience and monitoring of the antibody-mediated immune response to make recommendations prior to the collection of production bleeds.

Upon conclusion of the applied protocol, you are presented with the option of collecting a terminal bleed (this only applies to rabbits) or by extending your study for the collection of additional bleeds on a monthly basis.

Phase 3: Antibody Purification

After antibody development, we can use a number of different methods to purify your polyclonal antibodies.

• Protein A purification
• Protein G purification
• Protein A/G purification
• Affinity purification
• IgG purification
• IgY purification
• And more (i.e. BMP, gold, latex beads, etc.)

Phase 4: Antibody Conjugation

We provide a wide array of customizable conjugation services to meet specific assay and reagent needs.

Conjugates include the following:

• Biotin
• Horseradish peroxidase (HRP)
• Alkaline phosphatase (AP)
• Fluorescent dyes

Our Antigen Specialization

We are experts in generating antibodies specific for post-translational modifications— including phospho-epitopes, acetylation, methylation, hydroxylation, ubiquitinylation, and more—using modified peptides, and can optimize peptide design and synthesis as well as recombinant protein design and preparation.