Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP
Rat Monoclonal eB144
269 References
18-8817-30
18-8817-31
18-8817-33
20 µL
50 µL
200 µL
Liquid (sterile filtered)
Liquid (sterile filtered)
Liquid (sterile filtered)
WB, ELISA, IF, IP, Biochemical Assay, ChIP
Mouse
Rat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP - 18-8817-30
Anti-Mouse IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
Rat
Peroxidase (HRP) ULTRA
Monoclonal
IgG
Target Details
Mouse
Mouse TrueBlot® ULTRA Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA.
Application Details
ELISA, IP, WB
Biochemical Assay, ChIP, IF
- View References
Mouse TrueBlot® ULTRA has been tested in ELISA, IP, and Western blot and may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Mouse IgG TrueBlot® ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution factor for your desired application. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 8 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Formulation
1.0 mg/mL
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Proclin is added as an antimicrobial agent.
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Shipping & Handling
Wet Ice
Store at -20°C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.
Expiration date is six (6) months from date of receipt.
Mouse IgG TrueBlot® ULTRA is a unique Anti-Mouse IgG monoclonal secondary antibody. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western blot data with Mouse IgG TrueBlot® ULTRA, simply substitute the conventional Anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. Mouse IgG TrueBlot® ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Mouse IgG TrueBlot® ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot® ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
Lin LL et al. (2024). SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex. Nat Commun.
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WB, IB, PCA
Wang HH et al. (2024). Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders. J Clin Invest.
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WB, IB, PCA
Atarashi N et al. (2024). Activation of innate immune receptor TLR9 by mitochondrial DNA plays essential roles in the chemical long-term depression of hippocampal neurons. J Biol Chem.
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WB, IB, PCA
Kawahara K et al. (2024). Truncated GPNMB, a microglial transmembrane protein, serves as a scavenger receptor for oligomeric β-amyloid peptide1-42 in primary type 1 microglia. J Neurochem.
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WB, IB, PCA
Knowles S et al. (2024). Conditional Depletion of STN1 in Mouse Embryonic Fibroblasts. Bio Protoc.
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WB, IB, PCA
Maroni G et al. (2024). Tumor Microenvironment Landscapes Supporting EGFR-mutant NSCLC Are Modulated at the Single-cell Interaction Level by Unesbulin Treatment. Cancer Res Commun.
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WB, IB, PCA
Jones VT et al. (2024). Inhibition of autocrine HGF maturation overcomes cetuximab resistance in colorectal cancer. Cell Mol Life Sci.
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WB, IB, PCA
Cross T et al. (2023). RNA Profiles of Tear Fluid Extracellular Vesicles in Patients with Dry Eye-Related Symptoms. Int J Mol Sci.
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WB, IB, PCA
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WB, IB, PCA
Nguyen DD et al. (2023). Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair. Sci Adv.
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WB, IB, PCA
Dailey-Krempel B et al. (2023). A tug of war between DCC and ROBO1 signaling during commissural axon guidance. Cell Rep.
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WB, IB, PCA
Muñoz-Reyes D et al. (2023). The neuronal calcium sensor NCS-1 regulates the phosphorylation state and activity of the Gα chaperone and GEF Ric-8A. Elife.
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IP, Co-IP
Fang Z et al. (2023). Aurora A polyubiquitinates the BRCA1-interacting protein OLA1 to promote centrosome maturation. Cell Rep.
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WB, IB, PCA
Surana S et al. (2023). The tyrosine phosphatase LAR acts as a receptor of the nidogen-tetanus toxin complex. bioRxiv Preprint
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WB, IB, PCA
Jin D et al. (2023). α2δ‐1 protein drives opioid‐induced conditioned reward and synaptic NMDA receptor hyperactivity in the nucleus accumbens. J Neurochem.
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WB, IB, PCA
Pappas G et al. (2023). MDC1 maintains active elongation complexes of RNA polymerase II. Cell Rep.
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WB, IB, PCA
Gong L et al. (2023). AKT Phosphorylates FAM13A and Promotes Its Degradation via CUL4A/DDB1/DCAF1 E3 Complex. Am J Repair Cell Mol Biol.
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WB, IB, PCA
Boehm D et al. (2023). The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11. Cell Rep.
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WB, IB, PCA
Refaat AM et al. (2023). HNRNPU facilitates antibody class-switch recombination through C-NHEJ promotion and R-loop suppression. Cell Rep.
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WB, IB, PCA
Chauhan C et al. (2023). PRMT5-mediated regulatory arginine methylation of RIPK3. Cell Death Discov.
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WB, IB, PCA
Malgorzata Bodaszewska-Lubas et al. (2022). Dominant-Negative Form of SIGIRR: SIGIRRΔE8 Promotes Tumor Growth Through Regulation of Metabolic Pathways. J Interferon Cytokine Res.
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IP, Co-IP; WB, IB, PCA
Aso M et al. (2022). First-in-human autologous implantation of genetically modified adipocytes expressing LCAT for the treatment of familial LCAT deficiency. Heliyon.
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WB, IB, PCA
Blue SM et al. (2022). Transcriptome-wide identification of RNA-binding protein binding sites using seCLIP-seq. Nat Protoc.
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WB, IB, PCA
Amar-Schwartz A et al. (2022). S6K1 phosphorylates Cdk1 and MSH6 to regulate DNA repair. Elife.
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WB, IB, PCA
Janneh AH et al. (2022). Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis. Cell Rep.
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WB, IB, PCA
Park HJ et al. (2022). Anti‑inflammatory effects of Nypa fruticans Wurmb via NF‑κB and MAPK signaling pathways in macrophages. Exp Ther Med.
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WB, IB, PCA
Alerasool N et al. (2022). Identification and functional characterization of transcriptional activators in human cells. Mol Cel.
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WB, IB, PCA
Shen JZ et al. (2022). A FBXO7/EYA2-SCFFBXW7 axis promotes AXL-mediated maintenance of mesenchymal and immune evasion phenotypes of cancer cells. Mol Cell.
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WB, IB, PCA
Liang G et al. (2022). CTNNBL1 restricts HIV-1 replication by suppressing viral DNA integration into the cell genome. Cell Rep.
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WB, IB, PCA
Cha SJ et al. (2022). Therapeutic modulation of GSTO activity rescues FUS-associated neurotoxicity via deglutathionylation in ALS disease models. Dev Cell.
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WB, IB, PCA
Lahree A et al. (2022). Active APPL1 sequestration by Plasmodium favors liver-stage development. Cell Rep.
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WB, IB, PCA
Huang X et al. (2022). A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Cell Rep.
Applications
IP, Co-IP
Zeng LW et al. (2022). The membrane-associated ubiquitin ligases MARCH2 and MARCH3 target IL-5 receptor alpha to negatively regulate eosinophilic airway inflammation. Cell Mol Immunol.
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WB, IB, PCA
Volkmar N et al. (2022). Regulation of membrane fluidity by RNF145‐triggered degradation of the lipid hydrolase ADIPOR2. EMBO J.
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WB, IB, PCA
Desjardins P et al. (2022). The WNK1 kinase regulates the stability of transcription factors during wound healing of human corneal epithelial cells. J Cell Physiol.
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WB, IB, PCA
Namba Y et al. (2022). Maelstrom functions in the production of Siwi-piRISC capable of regulating transposons in Bombyx germ cells. iScience.
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WB, IB, PCA
Sarodaya N et al. (2022). Deubiquitinase USP19 enhances phenylalanine hydroxylase protein stability and its enzymatic activity. Cell Biol Toxicol.
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WB, IB, PCA
Saha B et al. (2022). Interactomic analysis reveals a homeostatic role for the HIV restriction factor TRIM5α in mitophagy. Cell Rep.
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WB, IB, PCA
Banerjee Sle et al. (2022). EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks. Cell Rep.
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WB, IB, PCA
Kucharski TJ et al. (2022). Small changes in phospho-occupancy at the kinetochore-microtubule interface drive mitotic fidelity. J Cell Biol.
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WB, IB, PCA
Sarodaya N et al. (2022). Deubiquitinase USP19 extends the residual enzymatic activity of phenylalanine hydroxylase variants. Sci Rep.
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WB, IB, PCA
Al-Habsi M et al. (2022). Spermidine activates mitochondrial trifunctional protein and improves antitumor immunity in mice. Science.
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WB, IB, PCA
McCool MA et al. (2022). Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing. RNA.
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WB, IB, PCA
Watanabe S et al. (2022). Skeletal muscle releases extracellular vesicles with distinct protein and microRNA signatures that function in the muscle microenvironment. PNAS Nexus.
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WB, IB, PCA
Walker AK et al. (2022). Modification of the SUMO activating enzyme subunit SAE2 directs SUMO isoform bias required for mitotic fidelity. bioRxiv Preprint
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WB, IB, PCA
Zhang, Z et al. (2022). Structure of SARS-CoV-2 membrane protein essential for virus assembly. Nature Communications
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WB, IB, PCA
Gao, Y et al. (2022). Pyrroloquinoline quinone (PQQ) protects mitochondrial function of HEI-OC1 cells under premature senescence. Npj Aging
Applications
IP, Co-IP
Xiang, Q et al. (2022). STAT and Janus kinase targeting by human herpesvirus 8 interferon regulatory factor in the suppression of type-I interferon signaling. PloS Pathogens
Applications
WB, IB, PCA
Lankford, C et al. (2022). Identification of HCN1 as a 14-3-3 client. PloS One
Applications
IP, Co-IP
Gayatri, MB et al. (2022). High glutamine suppresses osteogenesis through mTORC1-mediated inhibition of the mTORC2/AKT-473/RUNX2 axis. Cell Death Discovery
Applications
WB, IB, PCA; IP, Co-IP
Niewolik, D et al. (2022). Physical ARTEMIS:DNA-PKcs interaction is necessary for V(D)J recombination. Nucleic Acids Research
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WB, IB, PCA
Matsuda, Y et al. (2022). Phosphorylation of hTERT at threonine 249 is a novel tumor biomarker of aggressive cancer with poor prognosis in multiple organs. The Journal of Pathology
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IP, Co-IP
Papadopoulos D et al. (2022). MYCN recruits the nuclear exosome complex to RNA polymerase II to prevent transcription-replication conflicts. Mol Cell.
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WB, IB, PCA
Ambrosi G et al. (2022). Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells. Elife.
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WB, IB, PCA
Najafov A et al. (2021). RIPK1 Promotes Energy Sensing by the mTORC1 Pathway. Mol Cell
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WB, IB, PCA
Kaneya Y et al. (2021). Inhibitor for protein disulfide-isomerase family A member 3 enhances the antiproliferative effect of inhibitor for mechanistic target of rapamycin in liver cancer: An in vitro study on combination treatment with everolimus and 16F16. Oncol Lett.
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WB, IB, PCA
Alzhanova D et al. (2021). Novel modulators of p53-signaling encoded by unknown genes of emerging viruses. Plos Pathog
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WB, IB, PCA
Ruthnick D et al. (2021). The N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body. J Cell Biol
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WB, IB, PCA
Yang C et al. (2021). Kidney injury molecule-1 is a potential receptor for SARS-CoV-2. J Mol Cell Biol
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WB, IB, PCA
Zuliani I et al. (2021). The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome. Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics
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WB, IB, PCA
Zuliani I et al. (2021). High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer's Disease Signatures. International Journal of Molecular Sciences
Applications
IP, Co-IP; WB, IB, PCA
Ali SR et al. (2021). Angiocrine IGFBP3 spatially coordinates IGF signaling during neonatal cardiac regeneration. bioRxiv Preprint
Applications
WB, IB, PCA
Rojas-Lopez M et al. (2021). Pattern Recognition Receptor for Bacterial Lipopolysaccharide in the Cytosol of Human Macrophages. bioRxiv Preprint
Applications
WB, IB, PCA
Ali S et al. (2021). Angiocrine IGFBP3 spatially coordinates IGF signaling during neonatal cardiac regeneration. bioRxiv Preprint
Applications
WB, IB, PCA
Uchida Y et al. (2021). RLTPR Q575E: A novel recurrent gain‐of‐function mutation in patients with adult T‐cell leukemia/lymphoma. Eur J Haematol.
Applications
WB, IB, PCA
Joshi CS et al. (2021). NRF2 promotes urothelial cell response to bacterial infection by regulating reactive oxygen species and RAB27B expression. Cell Rep.
Applications
IP, Co-IP
Kumar A et al. (2021). Alanine Catabolism of Paneth Cells Maintains Intestinal Stem Cell Function During Dietary Restriction. Cell Press Preprint
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WB, IB, PCA
Sun Y et al. (2021). PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation. Nat Commun.
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WB, IB, PCA
Leppek K et al. (2021). VELCRO-IP RNA-seq reveals ribosome expansion segment function in translation genome-wide. Cell Rep.
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WB, IB, PCA
Wang J et al. (2021). Phase separation of OCT4 controls TAD reorganization to promote cell fate transitions. Cell Stem Cell.
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WB, IB, PCA
Metzger K et al. (2021). Effects of temperature on proliferation of myoblasts from donor piglets with different thermoregulatory maturities. BMC Mol Cell Biol.
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WB, IB, PCA
Kim J et al. (2021). Anti-adipocyte differentiation activity and flavonoid content determination by HPLC/UV analysis of tree sprouts. KoreaScience
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WB, IB, PCA
Ahn JJ et al. (2021). Protective effects of Camellia sinensis fruit and fruit peels against oxidative DNA damage. KoreaScience
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WB, IB, PCA
Jang TW et al. (2021). Anti-Inflammatory Effects of Abeliophyllum distichum Nakai (Cultivar Okhwang 1) Callus through Inhibition of PI3K/Akt, NF-κB, and MAPK Signaling Pathways in Lipopolysaccharide-Induced Macrophages. Processes.
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WB, IB, PCA
Matsuda S et al. (2021). Subunit-dependent and independent rules of AMPA receptor trafficking during long-term depression in hippocampal neurons. J Biol Chem.
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WB, IB, PCA
Nakayama A et al. (2021). Characterisation of a novel KRAS G12C inhibitor ASP2453 that shows potent anti-tumour activity in KRAS G12C-mutated preclinical models. Br J Cancer.
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WB, IB, PCA
Li X et al. (2021). CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression. Autophagy.
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WB, IB, PCA
Kawashima T et al. (2021). Phosphorylation of Collapsin Response Mediator Protein 1 (CRMP1) at Tyrosine 504 residue regulates Semaphorin 3A‐induced cortical dendritic growth. J Neurochem.
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WB, IB, PCA
Hazan R et al. (2021). E2F4's cytoplasmic role in multiciliogenesis is mediated via an N-terminal domain that binds two components of the centriole replication machinery, Deup1 and SAS6. Mol Biol Cell.
Applications
WB, IB, PCA
Piette BL et al. (2021). Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains. Mol Cell.
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WB, IB, PCA
Higa M et al. (2021). TRF2-mediated ORC recruitment underlies telomere stability upon DNA replication stress. Nucleic Acids Res.
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WB, IB, PCA
Lanzillotta C et al. (2021). Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention. Prog Neurobiol.
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WB, IB, PCA
Tavernier N et al. (2021). Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry. Nat Commun.
Applications
WB, IB, PCA
Lankford C et al. (2021). Identification of HCN1 as a 14-3-3 client. bioRxiv Preprint
Applications
WB, IB, PCA
Ueda K et al. (2021). MDMX acts as a pervasive preleukemic-to-acute myeloid leukemia transition mechanism. Cancer Cell.
Applications
WB, IB, PCA
Banerjee SL et al. (2021). Proximity proteomics reveal EPH tyrosine kinase receptors' regulation of cell polarity and identify Par3 scaffold protein as a novel effector of EPH signaling. bioRxiv Preprint
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WB, IB, PCA
Iwakawa HO et al. (2021). Ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates the production of secondary siRNAs in plants. Cell Rep.
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WB, IB, PCA
Rojas-Lopez M et al. (2021). Cytosolic Sensor of Bacterial Lipopolysaccharide in Human Macrophages. bioRxiv Preprint
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WB, IB, PCA
Toyomoto M et al. (2021). S1PR3–G12-biased agonist ALESIA targets cancer metabolism and promotes glucose starvation. Cell Chem Biol.
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WB, IB, PCA
Lohani S et al. (2021). A novel role of PRL in regulating epithelial cell density by inducing apoptosis at confluence. J Cell Sci.
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WB, IB, PCA
Moore J et al. (2021). Random errors in protein synthesis activate an age-dependent program of muscle atrophy in mice. Commun Biol.
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WB, IB, PCA
Alzhanova D et al. (2021). The ORF45 Protein of Kaposi Sarcoma-Associated Herpesvirus Is an Inhibitor of p53 Signaling during Viral Reactivation. J Virol.
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WB, IB, PCA
Kucharski TJ et al. (2021). Chromosome segregation fidelity is controlled by small changes in phospho-occupancy at the kinetochore-microtubule interface. bioRxiv Preprint
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WB, IB, PCA
Ukaji T et al. (2021). Novel knock‐in mouse model for the evaluation of the therapeutic efficacy and toxicity of human podoplanin–targeting agents. Cancer Science
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WB, IB, PCA
He X et al. (2021). Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche. Cell Rep.
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WB, IB, PCA
Hitachi K et al. (2021). Myoparr-associated and-independent multiple roles of heterogeneous nuclear ribonucleoprotein k during skeletal muscle cell differentiation. Int J Mol Sci.
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WB, IB, PCA
Miyagi E et al. (2020). Inhibition of Vif-Mediated Degradation of APOBEC3G through Competitive Binding of Core-Binding Factor Beta. J Virol
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WB, IB, PCA
Pereira C et al. (2020). Nuclear Accumulation of LAP1: TRF2 Complex during DNA Damage Response Uncovers a Novel Role for LAP1. Cells
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WB, IB, PCA
Lewis A et al. (2020). A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi. Mol Cell
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WB, IB, PCA
Tsukiyama T et al. (2020). A phospho-switch controls RNF43-mediated degradation of Wnt receptors to suppress tumorigenesis. Nat Commun
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WB, IB, PCA
Fuentes-Iglesias A et al. (2020). An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro. STAR Protoc.
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WB, IB, PCA
Nilsson J et al. (2020). NKT cells promote both type 1 and type 2 inflammatory responses in a mouse model of liver fibrosis. Sci Rep.
Applications
WB, IB, PCA
Lear et al. (2020). Kelch-like protein 42 is a pro-fibrotic ubiquitin E3 ligase involved in in systemic sclerosis. Journal of Biological Chemistry
Applications
IP, Co-IP; WB, IB, PCA
Lear et al. (2020). Kelch-like protein 42 is a pro-fibrotic ubiquitin E3 ligase involved in in systemic sclerosis. Journal of Biological Chemistry
Applications
WB, IB, PCA
Jang, T. et al. (2020). Protective and inhibitory effects of acteoside from Abeliophyllum distichum Nakai against oxidative DNA damage. Molecular Medicine Reports
Applications
WB, IB, PCA
Zhou Q, Cheng C, Wei Y, et al. (2020). USP15 potentiates NF-κB activation by differentially stabilizing TAB2 and TAB3. FEBS J.
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WB, IB, PCA
Kwan SY, Au-Yeung CL, Yeung TL, et al. (2020). Ubiquitin Carboxyl-Terminal Hydrolase L1 (UCHL1) Promotes Uterine Serous Cancer Cell Proliferation and Cell Cycle Progression. Cancers (Basel).
Applications
IP, Co-IP
Li Q, Sun Y, Jarugumilli GK, et al. (2020). Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription. Cell Stem Cell.
Applications
Undefined
Zhou H, Blevins MA, Hsu JY, et al. (2020). Identification of a Small-Molecule Inhibitor That Disrupts the SIX1/EYA2 Complex, EMT, and Metastasis. Cancer Res.
Applications
WB, IB, PCA
Bacon CW, Challa A, Hyder U, et al. (2020). KAP1 Is a Chromatin Reader that Couples Steps of RNA Polymerase II Transcription to Sustain Oncogenic Programs. Mol Cell.
Applications
IP, Co-IP
Rosàs-Canyelles E, Modzelewski AJ, Geldert A, He L, Herr AE. (2020). Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design. Sci Adv.
Applications
WB, IB, PCA
Jang TW, Choi JS, Park JH. (2020). Protective and inhibitory effects of acteoside from Abeliophyllum distichum Nakai against oxidative DNA damage. Mol Med Rep.
Applications
WB, IB, PCA
Wuerth JD, Habjan M, Kainulainen M, et al. (2020). eIF2B as a Target for Viral Evasion of PKR-Mediated Translation Inhibition. mBio.
Applications
WB, IB, PCA
Wang Q, Zhang T, Chang X, et al. (2020). Targeting Opsin4/Melanopsin with a Novel Small Molecule Suppresses PKC/RAF/MEK/ERK Signaling and Inhibits Lung Adenocarcinoma Progression. Mol Cancer Res.
Applications
WB, IB, PCA
Wheeler EC, Vu AQ, Einstein JM, et al. (2020). Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors. Nat Methods.
Applications
WB, IB, PCA
Johnson JS, De Veaux N, Rives AW, et al. (2020). A Comprehensive Map of the Monocyte-Derived Dendritic Cell Transcriptional Network Engaged upon Innate Sensing of HIV. Cell Rep.
Applications
WB, IB, PCA
Bie AS, Cömert C, Körner R, et al. (2020). An inventory of interactors of the human HSP60/HSP10 chaperonin in the mitochondrial matrix space. Cell Stress Chaperones.
Applications
WB, IB, PCA
Brekk OR, Korecka JA, Crapart CC, et al. (2020). Upregulating β-hexosaminidase activity in rodents prevents α-synuclein lipid associations and protects dopaminergic neurons from α-synuclein-mediated neurotoxicity. Acta Neuropathol Commun.
Applications
WB, IB, PCA
Lin VJT, Hu J, Zolekar A, Yan LJ, Wang YC. (2020). Urine Sample-Derived Cerebral Organoids Suitable for Studying Neurodevelopment and Pharmacological Responses. Front Cell Dev Biol.
Applications
IP, Co-IP
Msaouel P et al. (2020). Comprehensive molecular characterization identifies distinct genomic and immune hallmarks of renal medullary carcinoma. Cancer Cell.
Applications
WB, IB, PCA
Kubo Y et al. (2020). Periostin and tenascin-C interaction promotes angiogenesis in ischemic proliferative retinopathy. Sci Rep.
Applications
IP, Co-IP; WB, IB, PCA
Huang T et al. (2020). Fbxo7 and Pink1 play a reciprocal role in regulating their protein levels. Aging (Albany NY).
Applications
WB, IB, PCA
Joshi CS et al. (2020). Oxidative Stress Culminates in Intracellular Bacteria Expulsion Via NRF2/KEAP1 Induction of RAB27B. Cell Press Preprint
Applications
WB, IB, PCA
Liu Y et al. (2020). WSTF acetylation by MOF promotes WSTF activities and oncogenic functions. Oncogene.
Applications
Undefined
Leppek K et al. (2020). Gene-and Species-Specific hox mRNA translation by ribosome expansion segments. Mol Cell.
Applications
WB, IB, PCA
Ko JH et al. (2020). TREX2 Exonuclease Causes Spontaneous Mutations and Stress-Induced Replication Fork Defects in Cells Expressing RAD51K133A. Cell Rep.
Applications
WB, IB, PCA
Dolicka D et al. (2020). Tristetraprolin promotes hepatic inflammation and tumor initiation but restrains cancer progression to malignancy. Cell Mol Gastroenterol Hepatol.
Applications
WB, IB, PCA
Tian X et al. (2020). Inhibiting calpain 1 and 2 in cyclin G associated kinase–knockout mice mitigates podocyte injury. JCI Insight.
Applications
WB, IB, PCA
Karam N et al. (2019). Bone-Specific Overexpression of PITX1 Induces Senile Osteoporosis in Mice Through Deficient Self-Renewal of Mesenchymal Progenitors and Wnt Pathway Inhibition. Sci Rep.
Applications
WB, IB, PCA
Mehto et al. (2019). The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Molecular Cell
Applications
WB, IB, PCA
Mehto et al. (2019). The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Molecular Cell
Applications
IP, Co-IP
Graves-Deal et al. (2019). Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer. Oncotarget
Applications
WB, IB, PCA
Zhao et al. (2019). Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5. Nature Communications
Applications
IP, Co-IP
Islam H, Kobayashi M, Honjo T. (2019). Apurinic/apyrimidinic endonuclease 1 (APE1) is dispensable for activation-induced cytidine deaminase (AID)-dependent somatic hypermutation in the immunoglobulin gene. Int Immunol.
Applications
WB, IB, PCA
Rueda EM, Hall BM, Hill MC, et al. (2019). The Hippo Pathway Blocks Mammalian Retinal Müller Glial Cell Reprogramming. Cell Rep.
Applications
IP, Co-IP
Crotti A, Sait HR, McAvoy KM, et al. (2019). BIN1 favors the spreading of Tau via extracellular vesicles. Sci Rep.
Applications
WB, IB, PCA
Crotti A, Sait HR, McAvoy KM, et al. (2019). BIN1 favors the spreading of Tau via extracellular vesicles. Sci Rep.
Applications
WB, IB, PCA; IP, Co-IP
Meyer SN, Scuoppo C, Vlasevska S, et al. (2019). Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma. Immunity.
Applications
WB, IB, PCA
Santos M, Morais S, Pereira C, Sequeiros J, Alonso I. (2019). Parkin truncating variants result in a loss-of-function phenotype. Sci Rep.
Applications
WB, IB, PCA; IP, Co-IP
Li H, Li Q, Dang K, et al. (2019). YAP/TAZ Activation Drives Uveal Melanoma Initiation and Progression. Cell Rep.
Applications
WB, IB, PCA
Andersen L, Gülich AF, Alteneder M, et al. (2019). The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3+ Regulatory T Cells. Cell Rep.
Applications
WB, IB, PCA
Dick J.H. van den Boomen, Agata Sienkiewicz, Ilana Berlin, Marlieke L.M. Jongsma, Daphne M. van Elsl (2019). A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export. Nat Commun
Applications
IF, Confocal Microscopy
Yang-Hartwich Y, Tedja R, Roberts CM, et al. (2019). p53-Pirh2 Complex Promotes Twist1 Degradation and Inhibits EMT. Mol Cancer Res.
Applications
WB, IB, PCA
Tedja R, Roberts CM, Alvero AB, et al. (2019). Protein kinase Cα-mediated phosphorylation of Twist1 at Ser-144 prevents Twist1 ubiquitination and stabilizes it. J Biol Chem.
Applications
WB, IB, PCA
Najafov A, Mookhtiar AK, Luu HS, et al. (2019). TAM Kinases Promote Necroptosis by Regulating Oligomerization of MLKL. Mol Cell.
Applications
WB, IB, PCA
Le-Bel G, Cortez Ghio S, Guérin LP, Bisson F, Germain L, Guérin SL. (2019). Irradiated Human Fibroblasts as a Substitute Feeder Layer to Irradiated Mouse 3T3 for the Culture of Human Corneal Epithelial Cells: Impact on the Stability of the Transcription Factors Sp1 and NFI. Int J Mol Sci.
Applications
WB, IB, PCA
Boonying W, Joselin A, Huang E, et al. (2019). Pink1 regulates FKBP5 interaction with AKT/PHLPP and protects neurons from neurotoxin stress induced by MPP. J Neurochem.
Applications
WB, IB, PCA
Paoletti A, Allouch A, Caillet M, et al. (2019). HIV-1 Envelope Overcomes NLRP3-Mediated Inhibition of F-Actin Polymerization for Viral Entry. Cell Rep.
Applications
WB, IB, PCA
Kumanishi S, Yamanegi K, Nishiura H, et al. (2019). Epigenetic modulators hydralazine and sodium valproate act synergistically in VEGI-mediated anti-angiogenesis and VEGF interference in human osteosarcoma and vascular endothelial cells. Int J Oncol.
Applications
WB, IB, PCA
Marcar L, Bardhan K, Gheorghiu L, et al. (2019). Acquired Resistance of EGFR-Mutated Lung Cancer to Tyrosine Kinase Inhibitor Treatment Promotes PARP Inhibitor Sensitivity. Cell Rep.
Applications
WB, IB, PCA
Pattschull G, Walz S, Gründl M, et al. (2019). The Myb-MuvB Complex Is Required for YAP-Dependent Transcription of Mitotic Genes. Cell Rep.
Applications
WB, IB, PCA
Shimoda T et al. (2019). Expression of protein disulfide isomerase A3 and its clinicopathological association in gastric cancer. Oncol Rep.
Applications
WB, IB, PCA
Hwang JY et al. (2019). Dual Sensing of Physiologic pH and Calcium by EFCAB9 Regulates Sperm Motility. Cell.
Applications
WB, IB, PCA
Liu et al. (2018). Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression. Molecular Cell
Applications
WB, IB, PCA
Pasquin et al. (2018). Effect of human very low-density lipoproteins on cardiotrophin-like cytokine factor 1 (CLCF1) activity. Scientific Reports
Applications
IP, Co-IP
Guarnieri et al. (2018). The miR-106b-25 cluster mediates breast tumor initiation through activation of NOTCH1 via direct repression of NEDD4L. Oncogene
Applications
IP, Co-IP
Park et al. (2018). ATP depletion during mitotic arrest induces mitotic slippage and APC/CCdh1-dependent cyclin B1 degradation. Experimental & Molecular Medicine
Applications
IP, Co-IP; WB, IB, PCA
Mitchell et al. (2018). IL1RAP potentiates multiple oncogenic signaling pathways in AML. Journal of Experimental Medicine
Applications
IP, Co-IP
Brekk et al. (2018). Lipid-dependent deposition of alpha-synuclein and Tau on neuronal Secretogranin II-positive vesicular membranes with age. Scientific Reports
Applications
WB, IB, PCA
Jeon et al. (2018). PDI Knockdown Inhibits Seizure Activity in Acute Seizure and Chronic Epilepsy Rat Models via S-Nitrosylation-Independent Thiolation on NMDA Receptor. Frontiers in Cellular Neuroscience
Applications
WB, IB, PCA
Menzies et al. (2018). The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. Elife
Applications
IP, Co-IP
D'Souza et al. (2018). Gankyrin Promotes Tumor-Suppressor Protein Degradation to Drive Hepatocyte Proliferation. Cellular and Molecular Gastroenterology and Hepatology
Applications
WB, IB, PCA
Kistler et al. (2018). Phase transitioned nuclear Oskar promotes cell division of Drosophila primordial germ cells. Elife
Applications
WB, IB, PCA
Ikeuchi et al. (2018). Extracellular Protein Fibulin-7 and Its C-Terminal Fragment Have In Vivo Antiangiogenic Activity. Scientific Reports
Applications
WB, IB, PCA
Kumar A, Giri S, Shaha C. (2018). Sestrin2 facilitates glutamine-dependent transcription of PGC-1α and survival of liver cancer cells under glucose limitation. FEBS J.
Applications
WB, IB, PCA; IP, Co-IP
Wang Z, Yang B, Zhang M, et al. (2018). lncRNA Epigenetic Landscape Analysis Identifies EPIC1 as an Oncogenic lncRNA that Interacts with MYC and Promotes Cell-Cycle Progression in Cancer. Cancer Cell.
Applications
WB, IB, PCA
Dai L, Zhao T, Bisteau X, et al. (2018). Modulation of Protein-Interaction States through the Cell Cycle. Cell.
Applications
IP, Co-IP
Choi SW, Park HH, Kim S, et al. (2018). PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation. Mol Cell.
Applications
WB, IB, PCA; IP, Co-IP
Ghezraoui H, Oliveira C, Becker JR, et al. (2018). 53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ. Nature.
Applications
WB, IB, PCA
Brescia P, Schneider C, Holmes AB, et al. (2018). MEF2B Instructs Germinal Center Development and Acts as an Oncogene in B Cell Lymphomagenesis. Cancer Cell.
Applications
WB, IB, PCA
Hayashi K, Taura M, Iwasaki A. (2018). The interaction between IKKα and LC3 promotes type I interferon production through the TLR9-containing LAPosome. Sci Signal.
Applications
WB, IB, PCA
Yao B, Li Y, Wang Z, et al. (2018). Active N6-Methyladenine Demethylation by DMAD Regulates Gene Expression by Coordinating with Polycomb Protein in Neurons. Mol Cell.
Applications
WB, IB, PCA
Kitamura K, Que L, Shimadu M, et al. (2018). Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus. PLoS Pathog.
Applications
WB, IB, PCA
Hou W, Cruz-Cosme R, Wen F, et al. (2018). Expression of Human Cytomegalovirus IE1 Leads to Accumulation of Mono-SUMOylated PML That Is Protected from Degradation by Herpes Simplex Virus 1 ICP0. J Virol.
Applications
WB, IB, PCA
Lahaye X, Gentili M, Silvin A, et al. (2018). NONO Detects the Nuclear HIV Capsid to Promote cGAS-Mediated Innate Immune Activation. Cell. 2018;175(2):488-501.e22. doi:10.1016/j.cell.2018.08.062 Cell.
Applications
WB, IB, PCA
Monzón-Casanova E, Screen M, Díaz-Muñoz MD, et al. (2018). The RNA-binding protein PTBP1 is necessary for B cell selection in germinal centers. Nat Immunol.
Applications
WB, IB, PCA
Maeda T, Suetake H, Odaka T, Miyadai T. (2018). Original Ligand for LTβR Is LIGHT: Insight into Evolution of the LT/LTβR System. J Immunol.
Applications
WB, IB, PCA; In Vitro Binding Assay
Lampert F, Stafa D, Goga A, et al. (2018). The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation. Elife.
Applications
WB, IB, PCA
Garrido F, Pacheco M, Vargas-Martínez R, et al. (2018). Identification of hepatic protein-protein interaction targets for betaine homocysteine S-methyltransferase. PLoS One.
Applications
WB, IB, PCA
Min Y, Kim MJ, Lee S, Chun E, Lee KY. (2018). Inhibition of TRAF6 ubiquitin-ligase activity by PRDX1 leads to inhibition of NFKB activation and autophagy activation. Autophagy.
Applications
WB, IB, PCA
Seidi A, Muellner-Wong LS, Rajendran E, et al. (2018). Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presence of a divergent cytochrome c oxidase. Elife.
Applications
WB, IB, PCA
Mori M, Hazan R, Danielian PS, et al. (2018). Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. Nat Commun.
Applications
WB, IB, PCA
Zhang et al. (2017). Protein Phosphotyrosine Phosphatase 1B (PTP1B) in Calpain-dependent Feedback Regulation of Vascular Endothelial Growth Factor Receptor (VEGFR2) in Endothelial Cells: IMPLICATIONS IN VEGF-DEPENDENT ANGIOGENESIS AND DIABETIC WOUND HEALING. Journal of Biological Chemistry
Applications
IP, Co-IP
Rivera-Serrano et al. (2017). A Cytoplasmic RNA Virus Alters the Function of the Cell Splicing Protein SRSF2. Journal of Virology
Applications
IP, Co-IP
Mansilla et al. (2017). Interference of the complex between NCS-1 and Ric8a with phenothiazines regulates synaptic function and is an approach for fragile X syndrome. Proc. Natl. Acad. Sci. U.S.A
Applications
IP, Co-IP; WB, IB, PCA
Nounamo et al. (2017). An interaction domain in human SAMD9 is essential for myxoma virus host-range determinant M062 antagonism of host anti-viral function. Virology
Applications
IP, Co-IP; WB, IB, PCA
Zhai et al. (2017). NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene
Applications
IP, Co-IP; WB, IB, PCA
Bourens et al. (2017). Human mitochondrial cytochrome c oxidase assembly factor COX18 acts transiently as a membrane insertase within the subunit 2 maturation module. Journal of Biological Chemistry
Applications
IP, Co-IP; WB, IB, PCA
Hu et al. (2017). An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice. Cell Stem Cell
Applications
IF, Confocal Microscopy; WB, IB, PCA
Lewis et al. (2017). RNA Binding Protein CUGBP1 Inhibits Liver Cancer in a Phosphorylation-Dependent Manner. Molecular and Cellular Biology
Applications
WB, IB, PCA
Bononi et al. (2017). BAP1 regulates IP3R3-mediated Ca2+ flux to mitochondria suppressing cell transformation. Nature
Applications
WB, IB, PCA
Mori et al. (2017). Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. Nature Communications
Applications
IP, Co-IP
Joachim et al. (2017). Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Current Biology
Applications
IP, Co-IP
Miller et al. (2017). A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication. Cell Host Microbe
Applications
WB, IB, PCA
Miller et al. (2017). A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication. Cell Host Microbe
Applications
IF, Confocal Microscopy
Weng et al. (2017). POPX2 phosphatase regulates apoptosis through the TAK1-IKK-NF-κB pathway. Cell Death & Disease
Applications
WB, IB, PCA
Hiyama et al. (2017). Blue light and CO2 signals converge to regulate light-induced stomatal opening. Nature Communications
Applications
IP, Co-IP; WB, IB, PCA
Lehmann et al. (2017). Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1. Molecular Cell
Applications
IP, Co-IP
Lu et al. (2017). Cell cycle-dependent phosphorylation regulates RECQL4 pathway choice and ubiquitination in DNA double-strand break repair. Nature Communications
Applications
IP, Co-IP; WB, IB, PCA
Seong et al. (2017). Zinc finger protein ZPR9 functions as an activator of AMPK-related serine/threonine kinase MPK38/MELK involved in ASK1/TGF-β/p53 signaling pathways. Scientific Reports
Applications
IP, Co-IP; WB, IB, PCA
Kashihara et al. (2017). Angiotensin II activates CaV1.2 Ca2+channels through β-arrestin2 and casein kinase 2 in mouse immature cardiomyocytes. The Journal of Physiology
Applications
WB, IB, PCA
Torregrosa-Muñumer R, Forslund JME, Goffart S, et al. (2017). PrimPol is required for replication reinitiation after mtDNA damage. Proc Natl Acad Sci U S A.
Applications
WB, IB, PCA
Reitsma JM, Liu X, Reichermeier KM, et al. (2017). Composition and Regulation of the Cellular Repertoire of SCF Ubiquitin Ligases. Cell.
Applications
WB, IB, PCA
Saud K, Cánovas J, Lopez CI, et al. (2017). SFPQ associates to LSD1 and regulates the migration of newborn pyramidal neurons in the developing cerebral cortex. Int J Dev Neurosci.
Applications
WB, IB, PCA
Simsek D, Tiu GC, Flynn RA, et al. (2017). The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell.
Applications
WB, IB, PCA
Iwahori S, Umaña AC, VanDeusen HR, Kalejta RF. (2017). Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins. J Biol Chem.
Applications
WB, IB, PCA
Iwahori S, Kalejta RF. (2017). Phosphorylation of transcriptional regulators in the retinoblastoma protein pathway by UL97, the viral cyclin-dependent kinase encoded by human cytomegalovirus. Virology.
Applications
WB, IB, PCA
Perez KJ, Martínez FP, Cosme-Cruz R, Perez-Crespo NM, Tang Q. Correction for Perez et al., (2017). A Short cis-Acting Motif in the M112-113 Promoter Region Is Essential for IE3 To Activate M112-113 Gene Expression and Is Important for Murine Cytomegalovirus Replication. J Virol.
Applications
WB, IB, PCA
Roncero et al. (2016). Contribution of JAK2 mutations to T-cell lymphoblastic lymphoma development. Leukemia
Applications
IP, Co-IP
Vijayalingam et al. (2016). The Cellular Protein Complex Associated with a Transforming Region of E1A Contains c-MYC. Journal of Virology
Applications
WB, IB, PCA
Vijayalingam et al. (2016). The Cellular Protein Complex Associated with a Transforming Region of E1A Contains c-MYC. Journal of Virology
Applications
WB, IB, PCA; IP, Co-IP
Suzuki et al. (2016). Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication. PLOS Pathogens
Applications
WB, IB, PCA
Narusaka et al. (2016). Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1. Scientific Reports
Applications
IP, Co-IP; WB, IB, PCA
Kenific et al. (2016). NBR1 enables autophagy-dependent focal adhesion turnover. Journal of Cell Biology
Applications
IP, Co-IP
Saito et al. (2016). Oral administration of Aloe vera gel powder prevents UVB-induced decrease in skin elasticity via suppression of overexpression of MMPs in hairless mice. Bioscience, Biotechnology, and Biochemistry
Applications
WB, IB, PCA
Zhang et al. (2016). Direct Demonstration That Loop1 of Scap Binds to Loop7: A CRUCIAL EVENT IN CHOLESTEROL HOMEOSTASIS. Journal of Biological Chemistry
Applications
WB, IB, PCA
Akiyama et al. (2016). Cyclic Nucleotide Control of Microtubule Dynamics for Axon Guidance. The Journal of Neuroscience
Applications
WB, IB, PCA
Granados-Durán et al. (2016). Complement system activation contributes to the ependymal damage induced by microbial neuraminidase. Journal of Neuroinflammation
Applications
WB, IB, PCA
Aly et al. (2016). RNA Exosome Complex Regulates Stability of the Hepatitis B Virus X-mRNA Transcript in a Non-stop-mediated (NSD) RNA Quality Control Mechanism. Journal of Biological Chemistry
Applications
WB, IB, PCA
Luo et al. (2016). SMG7 is a critical regulator of p53 stability and function in DNA damage stress response. Cell Discovery
Applications
WB, IB, PCA
Min et al. (2016). Cereblon negatively regulates TLR4 signaling through the attenuation of ubiquitination of TRAF6. Cell Death & Disease
Applications
WB, IB, PCA
McCormick et al. (2016). Suppressor of Cytokine Signaling (SOCS)1 Regulates Interleukin-4 (IL-4)-activated Insulin Receptor Substrate (IRS)-2 Tyrosine Phosphorylation in Monocytes and Macrophages via the Proteasome. Journal of Biological Chemistry
Applications
IP, Co-IP
Jungas et al. (2016). Eph-mediated tyrosine phosphorylation of citron kinase controls abscission. Journal of Cell Biology
Applications
IP, Co-IP; WB, IB, PCA
Jeganathan et al. (2016). Rac1-mediated cytoskeleton rearrangements induced by intersectin-1s deficiency promotes lung cancer cell proliferation, migration and metastasis. Molecular Cancer
Applications
IP, Co-IP; WB, IB, PCA
Urbanus et al. (2016). Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila. Molecular Systems Biology
Applications
WB, IB, PCA
Araki M, Yang Y, Masubuchi N, et al. (2016). Activation of the thrombopoietin receptor by mutant calreticulin in CALR-mutant myeloproliferative neoplasms. Blood.
Applications
WB, IB, PCA
Banerjee R, Russo N, Liu M, et al. : (2016). TRIP13 promotes error-prone nonhomologous end joining and induces chemoresistance in head and neck cancer. Nat Commun.
Applications
WB, IB, PCA
Masser DR, Clark NW, Van Remmen H, Freeman WM. (2016). Loss of the antioxidant enzyme CuZnSOD (Sod1) mimics an age-related increase in absolute mitochondrial DNA copy number in the skeletal muscle. Age (Dordr).
Applications
WB, IB, PCA
Pérez C, Pérez-Zúñiga FJ, Garrido F, Reytor E, Portillo F, Pajares MA. (2016). The Oncogene PDRG1 Is an Interaction Target of Methionine Adenosyltransferases. PLoS One.
Applications
WB, IB, PCA
Rohnalter V et al. (2015). A multi-stage process including transient polyploidization and EMT precedes the emergence of chemoresistent ovarian carcinoma cells with a dedifferentiated and pro-inflammatory secretory phenotype. Oncotarget
Applications
WB, IB, PCA
St-Denis et al. (2015). Myotubularin-related proteins 3 and 4 interact with polo-like kinase 1 and centrosomal protein of 55 kDa to ensure proper abscission. Molecular & Cellular Proteomics
Applications
IP, Co-IP
Li et al. (2015). A monoclonal antibody that tracks endospore formation in the microsporidium Nosema bombycis. PLOS One
Applications
WB, IB, PCA
Gustin et al. (2015). Ebola Virus Glycoprotein Promotes Enhanced Viral Egress by Preventing Ebola VP40 From Associating With the Host Restriction Factor BST2/Tetherin. The Journal of Infectious Diseases
Applications
IP, Co-IP; WB, IB, PCA
Dagvadorj et al. (2015). Lipopolysaccharide Induces Alveolar Macrophage Necrosis via CD14 and the P2X7 Receptor Leading to Interleukin-1α Release. Immunity
Applications
IP, Co-IP
Greenfeld et al. (2015). TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation. PLOS Pathogens
Applications
WB, IB, PCA
Ibeawuchi et al. (2015). Hypertension-causing Mutations in Cullin3 Protein Impair RhoA Protein Ubiquitination and Augment the Association with Substrate Adaptors. Journal of Biological Chemistry
Applications
IP, Co-IP; WB, IB, PCA
Vuong et al. (2015). Differential Effects of Hepatocyte Nuclear Factor 4α Isoforms on Tumor Growth and T-Cell Factor 4/AP-1 Interactions in Human Colorectal Cancer Cells. Molecular and Cellular Biology
Applications
ChIP; WB, IB, PCA
Namba et al. (2015). Loss of p53 enhances the function of the endoplasmic reticulum through activation of the IRE1α/XBP1 pathway. Oncotarget
Applications
WB, IB, PCA
Sakaguchi et al. (2015). MAZR and Runx Factors Synergistically Repress ThPOK during CD8+ T Cell Lineage Development. Journal of Immunology
Applications
IP, Co-IP; WB, IB, PCA
Sakaguchi et al. (2015). MAZR and Runx Factors Synergistically Repress ThPOK during CD8+ T Cell Lineage Development. Journal of Immunology
Applications
IP, Co-IP
Lin et al. (2015). Expression of human carcinoembryonic antigen-related cell adhesion molecule 6 and alveolar progenitor cells in normal and injured lungs of transgenic mice. Physiological Reports
Applications
WB, IB, PCA
Liang G, Liu G, Kitamura K, et al. (2015). TGF-β suppression of HBV RNA through AID-dependent recruitment of an RNA exosome complex. PLoS Pathog.
Applications
WB, IB, PCA
Li H, Mohamed AA, Sharad S, et al. (2015). Silencing of PMEPA1 accelerates the growth of prostate cancer cells through AR, NEDD4 and PTEN. Oncotarget.
Applications
WB, IB, PCA
Iwahori S, Hakki M, Chou S, Kalejta RF. (2015). Molecular Determinants for the Inactivation of the Retinoblastoma Tumor Suppressor by the Viral Cyclin-dependent Kinase UL97. J Biol Chem.
Applications
WB, IB, PCA
Nishimura T. (2015). Role of extracellular matrix in development of skeletal muscle and postmortem aging of meat. Meat Sci.
Applications
WB, IB, PCA
Xu J, Eriksson SE, Cebula M, et al. (2015). The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death. Cell Death Dis.
Applications
WB, IB, PCA
Yousif AS et al. (2014). Differential regulation of S-region hypermutation and class switch recombination by noncanonical functions of uracil DNA glycosylase. Proc Natl Acad Sci USA
Applications
ChIP; IP, Co-IP
Puglisi R, Maccari I, Pipolo S, Mangia F, Boitani C. (2014). The nuclear form of glutathione peroxidase 4 colocalizes and directly interacts with protamines in the nuclear matrix during mouse sperm chromatin assembly. Spermatogenesis.
Applications
WB, IB, PCA
Jerman S, Ward HH, Lee R, et al. (2014). OFD1 and flotillins are integral components of a ciliary signaling protein complex organized by polycystins in renal epithelia and odontoblasts. PLoS One.
Applications
WB, IB, PCA
Petry FR, Pelletier J, Bretteville A, et al. (2014). Specificity of anti-tau antibodies when analyzing mice models of Alzheimer's disease: problems and solutions. PLoS One.
Applications
WB, IB, PCA
Liaunardy-Jopeace A, Bryant CE, Gay NJ. (2014). The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane. Sci Signal.
Applications
WB, IB, PCA
Gong H et al. (2013). Lipoprotein lipase (LPL) is associated with neurite pathology and its levels are markedly reduced in the dentate gyrus of Alzheimer's disease brains. The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Applications
WB, IB, PCA
Demir K, Kirsch N, Beretta CA, et al. (2013). RAB8B is required for activity and caveolar endocytosis of LRP6. Cell Rep.
Applications
WB, IB, PCA
Stuckenholz C, Lu L, Thakur PC, Choi TY, Shin D, Bahary N. (2013). Sfrp5 modulates both Wnt and BMP signaling and regulates gastrointestinal organogenesis [corrected] in the zebrafish, Danio rerio. PLoS One.
Applications
WB, IB, PCA
Ryan AS, Li G, Blumenthal JB, Ortmeyer HK. (2013). Aerobic exercise + weight loss decreases skeletal muscle myostatin expression and improves insulin sensitivity in older adults. Obesity (Silver Spring).
Applications
WB, IB, PCA
Murata H, Sakaguchi M, Kataoka K, Huh NH. (2013). SARM1 and TRAF6 bind to and stabilize PINK1 on depolarized mitochondria. Mol Biol Cell.
Applications
WB, IB, PCA
Nelersa CM et al. (2012). High-content analysis of proapoptotic EphA4 dependence receptor functions using small-molecule libraries. J Biolmol Screen
Applications
WB, IB, PCA
Yamagishi Y et al. (2012). MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components. Nat Cel Biol
Applications
WB, IB, PCA
Miyairi I, Ziebarth J, Laxton JD, et al. (2012). Host genetics and Chlamydia disease: prediction and validation of disease severity mechanisms. PLoS One.
Applications
IP, Co-IP
Martínez FP, Tang Q. (2012). Leucine zipper domain is required for Kaposi sarcoma-associated herpesvirus (KSHV) K-bZIP protein to interact with histone deacetylase and is important for KSHV replication. J Biol Chem.
Applications
WB, IB, PCA
Bonaterra GA, Zügel S, Thogersen J, et al. (2012). Growth differentiation factor-15 deficiency inhibits atherosclerosis progression by regulating interleukin-6-dependent inflammatory response to vascular injury. J Am Heart Assoc.
Applications
WB, IB, PCA
Cosme-Cruz R, Martínez FP, Perez KJ, Tang Q. (2011). H2B homology region of major immediate-early protein 1 is essential for murine cytomegalovirus to disrupt nuclear domain 10, but is not important for viral replication in cell culture. J Gen Virol.
Applications
WB, IB, PCA
Rzeczkowski K, Beuerlein K, Müller H, et al. (2011). c-Jun N-terminal kinase phosphorylates DCP1a to control formation of P bodies. J Cell Biol.
Applications
WB, IB, PCA
Decuypere JP, Welkenhuyzen K, Luyten T, et al. (2011). Ins(1,4,5)P3 receptor-mediated Ca2+ signaling and autophagy induction are interrelated. Autophagy.
Applications
WB, IB, PCA
Wang J, Zhang Y, Lu C, Lei L, Yu P, Zhong G. (2010). A genome-wide profiling of the humoral immune response to Chlamydia trachomatis infection reveals vaccine candidate antigens expressed in humans. J Immunol.
Applications
WB, IB, PCA
Kim S, Huang W, Mottillo EP, et al. (2010). Posttranslational regulation of membrane type 1-matrix metalloproteinase (MT1-MMP) in mouse PTEN null prostate cancer cells: Enhanced surface expression and differential O-glycosylation of MT1-MMP. Biochim Biophys Acta.
Applications
WB, IB, PCA
Prast-Nielsen S, Cebula M, Pader I, Arnér ES. (2010). Noble metal targeting of thioredoxin reductase--covalent complexes with thioredoxin and thioredoxin-related protein of 14 kDa triggered by cisplatin. Free Radic Biol Med.
Applications
WB, IB, PCA
Mitsuhashi S et al. (2008). Tautomycetin suppresses the TNFα/NF-κB pathway via inhibition of IKK activation. Int J Oncol
Applications
IP, Co-IP
Li H, Xu LL, Masuda K, et al. (2008). A feedback loop between the androgen receptor and a NEDD4-binding protein, PMEPA1, in prostate cancer cells. J Biol Chem.
Applications
WB, IB, PCA
What is Rockland’s TrueBlot® product line?
Rockland’s TrueBlot® product line is designed to solve common experimental problems when performing immunoprecipitation/co-immunoprecipitation experiments and Western blots of immunoprecipitated samples (IP–Western blot). The product line consists of TrueBlot® monoclonal secondary antibodies and TrueBlot® IP beads.
What is the advantage of TrueBlot® secondaries over regular secondary antibodies?
TrueBlot® antibodies are specific to the whole IgG molecule and will not bind heavy or light chains. This is useful for binding target proteins with an expected MW near 25 kDa (light chains) and 50 kDa (heavy chains).
Why does it appear that the TrueBlot® antibody is binding heavy/light chains in my sample?
The sample must be fully reduced to eliminate cross-reactivity with heavy and light chains. Any reactivity to heavy and light chains could be due to incomplete reduction of your sample. Please be sure to optimize reducing conditions for your sample type.
How can I ensure my samples are fully reduced?
Samples can be fully reduced by heating at 90-100°C for 10 minutes in sample buffer containing reducing agent (for example, DTT to a final concentration of 50 mM or add β-Mercaptoethanol to a final concentration of 2% (v/v)).
With which species/isotypes are TrueBlot® secondary antibodies reactive?
TrueBlot® secondary antibodies are reactive with the IgG of their respective species. For example, if using a primary antibody raised in mouse (mouse host), use TrueBlot® anti-mouse Ig secondary antibodies for detection.
Do TrueBlot® secondary antibodies detect IgM?
TrueBlot® secondary antibodies have not been shown to detect IgM.
Do I need to preclear my lysate before the immunoprecipitation step?
Samples that have many other proteins present (such as lysates) may require preclearing to prevent interference in later IP and WB procedures. Recombinant protein samples require pre-clearing more often than serum samples.
How can I enrich/concentrate my lysate for a low expression of a protein of interest?
The immunoprecipitation procedure can be repeated several times to yield a more concentrated protein solution.
What is the recommended lysis buffer for TrueBlot® products?
The optimal lysis buffer will depend on your sample type. See protocol for buffer recommendations. Generally, 1X RIPA is used to lyse samples.
My target protein has the same MW as a heavy/light chain. How can I be sure that the band is the target protein and not a heavy/light chain?
Be sure to include a positive control for the primary antibody in your experiment and check that the sample is fully reduced.
What is the average size of TrueBlot® magnetic/agarose beads?
The beads are roughly 0.5 μm in diameter.
Why can’t I use Protein A or Protein G beads for the IP step in IP–Western blot when using rabbit species?
Using Protein A or Protein G beads with rabbit species results in contaminated bands. For best results when using rabbit species, use Rockland’s TrueBlot® Rabbit Ig IP beads, which are available in either a magnetic or agarose format. Please see individual TrueBlot® Rabbit product pages for additional details.
Can I use Protein A or Protein G beads for the IP step in IP–Western blot when using mouse, goat, or sheep species?
Yes. If you are using Protein A or Protein G beads with mouse, goat, or sheep species, we recommend the following: Determine the compatibility of Protein A or Protein G with your species and subtype, do not use excessive amounts of slurry, and choose the appropriate elution conditions. In some instances, harsher elution conditions can cause stripping of the subunits of Protein A or Protein G from the bead support and result in non-specific bands. For best results, we recommend using TrueBlot® Ig IP beads, which are available in either agarose or magnetic format.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
