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Streptavidin
19 References
S000-01
5 mg
Lyophilized
ELISA, IF, FC, SDS-PAGE, LFA, Microarray, Other
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Streptavidin - S000-01
SA, S avidin, streptococcus avidin
15.5 U/mg by biotin titration method
Target Details
Streptavidin is chromatographically pure and shows predominantly a single 53,600 dalton band by SDS-PAGE.
P22629 - UniProtKB
CAA00084.1 - NCBI Protein
Application Details
SDS-PAGE
ELISA, FC, IF, Microarray, Other, LFA
- View References
Streptavidin has been tested by SDS-PAGE and is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized streptavidin.
Formulation
0.9 mg protein/mg streptavidin/lyophilizate (balance is sodium chloride).
0.15 M Sodium Chloride
None
None
5.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's affinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments.
Duan Y et al. (2023). Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand. Nat Biomed Eng.
Applications
EIA, ELISA
Li X et al. (2023). Polarized focal adhesion kinase activity within a focal adhesion during cell migration. NAt Chem Biol.
Applications
IF, Confocal Microscopy
Britain DM et al. (2022). Progressive enhancement of kinetic proofreading in T cell antigen discrimination from receptor activation to DAG generation. Elife.
Applications
IF, Confocal Microscopy
Blanchard, A et al. (2021). Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope. Nature Communications
Applications
Blocking
Blanchard A et al. (2021). Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope. Nat Commun.
Applications
IF, Confocal Microscopy
Lajkó, N et al. (2020). Sensitivity of Rodent Microglia to Kynurenines in Models of Epilepsy and Inflammation In Vivo and In Vitro: Microglia Activation is Inhibited by Kynurenic Acid and the Synthetic Analogue SZR104. International Journal of Molecular Sciences
Applications
IHC, ICC, Histology
Brockman, JM et al. (2020). Live-cell super-resolved PAINT imaging of piconewton cellular traction forces. Nature Methods
Applications
Blocking
Tischer, DK et al. (2019). Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signaling. ELife
Applications
Incubation
Brockman, JM et al. (2018). Mapping the 3D orientation of piconewton integrin traction forces. Nature Methods
Applications
Incubation
Zachary Crannell et al. (2016). Multiplexed Recombinase Polymerase Amplification Assay To Detect Intestinal Protozoa. Anal Chem.
Applications
Lateral Flow (LFA)
Lum, J et al. (2015). An Impedance Aptasensor with Microfluidic Chips for Specific Detection of H5N1 Avian Influenza Virus. Sensors (Basel, Switzerland)
Applications
Ab Immobilization
Janssen, K M J et al. (2015). Rheumatoid arthritis-associated autoantibodies in non-rheumatoid arthritis patients with mucosal inflammation: a case-control study. Arthritis Research & Therapy
Applications
Blocking
Ofori, LO et al. (2014). High-affinity recognition of HIV-1 frameshift-stimulating RNA alters frameshifting in vitro and interferes with HIV-1 infectivity. Journal of Medicinal Chemistry
Applications
SPR-Based Analyses
Jones MC et al. (2013). Characterization of cell seeding and specific capture of B cells in microbubble well arrays. Biomed Microdevices.
Applications
Microarray; Other
Hansen, SD et al. (2013). Cytoplasmic actin: purification and single molecule assembly assays. Methods in Molecular Biology (Clifton, N.J.)
Applications
Other
Hansen, SD et al. (2013). αE-catenin actin-binding domain alters actin filament conformation and regulates binding of nucleation and disassembly factors. Molecular Biology of the Cell
Applications
FC, FACS, FLOW
Bai, H et al. (2012). A SPR aptasensor for detection of avian influenza virus H5N1. Sensors (Basel, Switzerland)
Applications
SPR-Based Analyses
Lee, S et al. (2010). Optimized combination therapy using bortezomib, TRAIL and TLR agonists in established breast tumors. Cancer Immunology, Immunotherapy : Cii
Applications
Cellular assay
Haugland, GT. et al. (2006). Stimulation of MCM helicase activity by a Cdc6 protein in the archaeon Thermoplasma acidophilum. Nucleic Acids Research
Applications
Receptor Binding Assays
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
