Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) Staining Buffer with FBS

Datasheet

FACS and IF Staining Buffer with FBS

Name: FACS and IF Staining Buffer with FBS
Brand: Rockland Immunochemicals
Price: 87.0 USD
Availability: InStock

Item No. MB-086-0500

Size: 500 mL

Physical State: Liquid (sterile filtered)

Applications: IF, FC

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Specifications for FACS and IF Staining Buffer with FBS

Product Details

Description: Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) Staining Buffer with FBS - MB-086-0500

Synonyms: Fluorescent Activated Cell Sorting (FACS), Staining buffer, Flow cytometry staining buffer, Immunofluorescence staining buffer

Target Details

Purity/Specificity: FACS and IF Staining Buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Application Details

Tested Applications: FC, IF

Application Note: Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer is adequate for cell staining, washing and dilution in flow cytometry and immunofluorescence experiments.

Formulation

Concentration: 1X

Preservative: 0.1% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store container at 4° C prior to opening.

Expiration: Expiration date is three (3) months from date of receipt.

Background

Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer is a diluent buffer optimized for use in flow cytometry and immunofluorescence assay of viable and/or fixed cells. FACS and IF staining, washing buffer contains 2% calf bovine serum as carrier and stabilizer to reduce non-specific binding of antibodies and fluorochrome reagents to targets cells. Metabolic inhibitors and NaN3 are also included in the flow cytometry and immunofluorescence buffer to prevent patching, capping of surface antigen and bacterial growth.

Related Protocols

Flow Cytometry (FC) Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.