10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC Treated

Datasheet SDS

10X TE pH 7.5

Name: 10X TE pH 7.5
Brand: Rockland Immunochemicals
Price: 100.0 USD
Availability: InStock
Rating: 5 (1 reviews)

1 References

Item No. MB-006

Size: 1 L

Physical State: Liquid (sterile filtered)

Applications: IHC

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Specifications for 10X TE pH 7.5

Product Details

Description: 10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC Treated - MB-006

Synonyms: 10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) Solution, 10X TE pH 7.5 DEPC TREATED Solution

Target Details

Purity/Specificity: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Application Details

Suggested Applications: IHC - View References

Application Note: This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 100 mM Tris HCl and 10 mM EDTA at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in DEPC treated molecular biology grade water.

Formulation

Concentration: 10X

Buffer: See application note.

Preservative: None

Stabilizer: None

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.

Expiration: Expiration date is six (6) months from date of receipt.

Background

TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.

References (1)

De Jesus-Monge, WE. et al. (2010). Mismatch repair protein expression and colorectal cancer in Hispanics from Puerto Rico. Familial Cancer

Applications

IHC, ICC, Histology

PubMed

Related Protocols

Heat-induced Antigen Retrieval Protocol

Immunohistochemistry (IHC) Protocol

Protease-induced Antigen Retrieval Protocol

Staining Paraffin Sections by PAP Procedure

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.