10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20, pH 7.2)

Datasheet SDS

10X PBST

Name: 10X PBST
Brand: Rockland Immunochemicals
Price: 100.0 USD
Availability: InStock
Rating: 5 (2 reviews)

2 References

Item No. MB-075-1000

Size: 1 L

Physical State: Liquid

Applications: Microarray, Other

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Specifications for 10X PBST

Product Details

Description: 10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20, pH 7.2) - MB-075-1000

Synonyms: Phosphate Buffer Saline -Tween, Tween PBS

Target Details

Purity/Specificity: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Application Details

Suggested Applications: Microarray, Other - View References

Application Note: This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Dilute only prior to immediate use. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium Chloride and 0.5% (v/v) Tween-20 at a pH of 7.2. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.

Formulation

Concentration: 10X

Buffer: See application note.

Preservative: None

Stabilizer: None

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.

Expiration: Expiration date is six (6) months from date of receipt.

Background

Phosphate Buffer Saline-Tween is commonly use in washing procedure while performing western-blot, ELISA and, more generally immuno-assays.

References (2)

Zhang YP et al. (2023). Imaging Protein Aggregates in Parkinson's Disease Serum Using Aptamer-Assisted Single-Molecule Pull-Down. Anal Chem.

Applications

SiMPull

PubMed

Schwarz T et al. (2021). SARS-CoV-2 proteome-wide analysis revealed significant epitope signatures in COVID-19 patients. Front Immunol.

Applications

Other

PubMed

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.