Datasheet SDS

1.0 M Tris HCl pH 7.6

Name: 1.0 M Tris HCl pH 7.6
Brand: Rockland Immunochemicals
Price: 65.0 USD
Availability: InStock
Rating: 5 (5 reviews)

5 References

Item No. MB-003

Size: 1 L

Physical State: Liquid (sterile filtered)

Applications: Other

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Specifications for 1.0 M Tris HCl pH 7.6

Product Details

Description: 1.0 M Tris HCl pH 7.6 - MB-003

Synonyms: Tris solution, THAM, tris(hydroxymethyl)aminomethane

Target Details

Purity/Specificity: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Application Details

Suggested Applications: Other - View References

Application Note: Tris solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. Visit our newly expanded web site at www.rockland.com for methods using this and other buffers. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M Tris HCl at pH 7.6. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade (WFI) water.

Formulation

Concentration: 1.0M

Buffer: See application note.

Preservative: None

Stabilizer: None

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.

Expiration: Expiration date is six (6) months from date of receipt.

Background

Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.

References (5)

He Y et al. (2023). Diffusion-enhanced characterization of 3D chromatin structure reveals its linkage to gene regulatory networks and the interactome. Genome Res.

Applications

Cell Lysis

PubMed

Di L et al. (2022). Rapid and sensitive single-cell RNA sequencing with SHERRY2. BMC Biol.

Applications

RNA seq, qPCR, Reverse Transcription, RT-PCR Analysis

PubMed

Meyer M et al. (2021). Ebola vaccine-induced protection in nonhuman primates correlates with antibody specificity and Fc-mediated effects. Sci Transl Med.

Applications

EIA, ELISA

PubMed

Di L et al. (2020). RNA sequencing by direct tagmentation of RNA/DNA hybrids. Proc Natl Acad Sci USA.

Applications

Other

PubMed

Li R et al. (2020). A body map of somatic mutagenesis in morphologically normal human tissues. bioRxiv

Applications

Other

Source

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.