Datasheet SDS

0.5 M EDTA pH 8.0

Name: 0.5 M EDTA pH 8.0
Brand: Rockland Immunochemicals
Price: 90.0 USD
Availability: InStock
Rating: 5 (4 reviews)

4 References

Item No. MB-014

Size: 100 mL

Physical State: Liquid (sterile filtered)

Applications: FC

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Specifications for 0.5 M EDTA pH 8.0

Product Details

Description: 0.5 M EDTA pH 8.0 - MB-014

Synonyms: Ethylenediaminetetraacetic acid, EDTA

Application Details

Suggested Applications: FC - View References

Application Note: Ethylenediaminetetraacetic acid is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 0.5 M Trisodium EDTA at pH 8.0. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water (DI) treated with diethyl pyrocarbonate (DEPC).

Formulation

Concentration: 0.5M

Buffer: See application note.

Preservative: None

Stabilizer: None

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.

Expiration: Expiration date is six (6) months from date of receipt.

Background

EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins. In tissue culture EDTA is used as a chelating agent that binds to calcium and prevents joining of cadherins between cells, preventing clumping of cells grown in liquid suspension, or detaching adherent cells for passaging. EDTA is also known to inhibit a range of metallopeptidases, the method of inhibition occurs via the chelation of the metal ion required for catalytic activity.

References (4)

Henrick BM et al. (2021). Bifidobacteria-mediated immune system imprinting early in life. Cell.

Applications

FC, FACS, FLOW

PubMed

Lakshmikanth T et al. (2020). Human immune system variation during 1 year. Cell Rep.

Applications

FC, FACS, FLOW

PubMed

Rodriguez L et al. (2020). Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19 Cell Rep Med.

Applications

FC, FACS, FLOW

PubMed

Olin A et al. (2018). Stereotypic immune system development in newborn children. Cell.

Applications

FC, FACS, FLOW

PubMed

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.