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Swine IgM (mu) Antibody Peroxidase Conjugated
Rabbit Polyclonal
214-4307
20 mg
Lyophilized
Sus scrofa domesticus (Pig, Porcine, Swine)
Rabbit
This product is discontinued
Product Details
Rabbit Anti-Swine IgM (mu) Antibody Peroxidase Conjugated - 214-4307
rabbit anti-Swine IgM (mu chain) Peroxidase Conjugated Antibody, Swine Antibody, Rb-a-Swine IgG, Swine Antibody in Rabbit, Swine Secondary Antibody
Rabbit
IgM µ chain
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Sus scrofa domesticus (Pig, Porcine, Swine)
Native Protein
Anti-Swine IgM mu heavy chain was produced by repeated immunization with Swine IgM mu heavy chain in rabbit.
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgM and Swine Serum. No reaction was observed against Swine IgG.
Application Details
Dot Blot, ELISA
Anti-Swine IgM Peroxidase Conjugated Antibody has been tested by Dot blot and ELISA. This product has been assayed against 1.0 ug of Swine IgM in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Formulation
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Swine IgM mu heavy chain antibody generated in rabbit detects specifically Swine IgM mu heavy chain. This secondary antibody anti-Swine is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
