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Rat IgG (H&L) Antibody Biotin Conjugated
Goat Polyclonal
612-1602
2 mg
Lyophilized
WB, ELISA
Rat
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Goat Anti-Rat IgG (H&L) Antibody Biotin Conjugated - 612-1602
Goat anti-Rat IgG biotin Conjugated Antibody, Goat anti-Rat IgG Antibody biotin Conjugation
Goat
IgG (H&L)
Biotin
Polyclonal
IgG
Target Details
Rat
Rat IgG whole molecule
Anti-RAT IgG Biotin Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgG and Rat Serum.
Application Details
ELISA, WB
Anti-RAT IgG Biotin Conjugated antibody has been tested by ELISA and western blot and is suitable for immunoassays where specificity to the Rat immunoglobulin heavy and or light chain regions is desired. Anti-Rat antibody has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:500,000 is suggested for this product. Optimal concentrations in immunoassays should be determined by the researcher.
Formulation
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
RAT IgG Biotin Conjugated antibody detects rat immunoglobulin G. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
