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Rat IgG (H&L) Antibody ATTO 647N Conjugated Pre-Adsorbed
Goat Polyclonal
14 References
612-156-120S
612-156-120
100 µg
500 µg
Lyophilized
Lyophilized
WB, IHC, IF, Dot Blot, Multiplex, Other
Rat
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Goat Anti-Rat IgG (H&L) Antibody ATTO 647N Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) - 612-156-120
Goat anti-Rat IgG ATTO647N Conjugated Antibody, Goat anti-Rat IgG Antibody ATTO 647N Conjugation
Goat
IgG (H&L)
ATTO 647N
Polyclonal
IgG
Target Details
Rat
Rat IgG whole molecule
Rat IgG (H&L) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.
Application Details
Dot Blot, WB
IF, IHC, Other, Multiplex
- View References
Anti-Rat IgG (H&L) conjugated to ATTO 647N has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
500 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Rat IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Westeinde EA et al. (2024). Transforming a head direction signal into a goal-oriented steering command. Nature.
Applications
MultiColor FlpOut (MCFO)
Mellouk N et al. (2024). Post-translational targeting of Rab35 by the effector IcsB of Shigella determines intracellular bacterial niche formation. Cell Rep.
Applications
IF, Confocal Microscopy
Lillvis JL et al. (2024). Nested neural circuits generate distinct acoustic signals during Drosophila courtship. Curr Biol.
Applications
IF, Confocal Microscopy
Meissner GW wt al. (2023). A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution. Elife.
Applications
IF, Confocal Microscopy
Vijayan V et al. (2023). A rise-to-threshold process for a relative-value decision. Nature.
Applications
IF, Confocal Microscopy
Sun L et al. (2022). Recurrent circadian circuitry regulates central brain activity to maintain sleep. Neuron.
Applications
IF, Confocal Microscopy
Baker CA et al. (2022). Neural network organization for courtship-song feature detection in Drosophila. Curr Biol.
Applications
IHC, ICC, Histology
Okubo TS et al. (2020). A neural network for wind-guided compass navigation. Neuron.
Applications
IF, Confocal Microscopy; Other
Feng K et al. (2020). Distributed control of motor circuits for backward walking in Drosophila. Nat Commun.
Applications
IHC, ICC, Histology
Dolan MJ et al. (2019). Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body. Elife.
Applications
IF, Confocal Microscopy; IHC, ICC, Histology; Other
Wolff et al. (2018). Neuroarchitecture of the Drosophila central complex: A catalog of nodulus and asymmetrical body neurons and a revision of the protocerebral bridge catalog. The Journal of Comparative Neurology
Applications
WB, IB, PCA
Meissner et al. (2018). Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons. PLOS One
Applications
IHC, ICC, Histology; Multiplex Assay
Turner-Evans et al. (2017). Angular velocity integration in a fly heading circuit. Elife
Applications
IHC, ICC, Histology
von Reyn et al. (2017). Feature Integration Drives Probabilistic Behavior in the Drosophila Escape Response. Neuron
Applications
IF, Confocal Microscopy; Multiplex Assay
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
