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Rat IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated
Rabbit Polyclonal
612-4504
1 mg
Liquid (sterile filtered)
Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Rabbit Anti-Rat IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated - 612-4504
Rabbit Anti-Rat IgG F(ab')2 alkaline phosphatase Conjugated Antibody, Rabbit Anti-Rat IgG Fab2 alkaline phosphatase Conjugated Antibody, Rabbit Anti-Rat IgG Fab2 Fragment Antibody alk phos Conjugation
Rabbit
IgG F(ab')2
Alkaline Phosphatase (AP)
Polyclonal
IgG
Target Details
Rat
Rat IgG F(ab')2 fragment
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against Rat IgG F(c).
Application Details
This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,500 to 1:10,800 of the reconstitution concentration is suggested for this product.
Formulation
1.3 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Shipping & Handling
Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of receipt.
Anti-Rat IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the rat IgG molecule. Rat IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. F(ab')2 molecules lack the Fc portion of IgG and therefore receptors that bind rat IgG F(c) will not bind rat IgG F(ab')2 molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG F(ab')2 is conjugated to Alkaline Phosphatase.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
