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Rabbit IgG (H&L) Secondary Antibody DyLight™ 488 Conjugated
Goat Polyclonal
10 References
611-141-002
611-141-002-0.5
100 µg
5 x 100 µg
Lyophilized
Lyophilized
WB, IHC, IF, FC, Dot Blot, Multiplex
Rabbit
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Goat Anti-Rabbit IgG (H&L) Antibody DyLight™ 488 Conjugated - 611-141-002
Goat anti-Rabbit IgG Antibody DyLight™488 Conjugation, Goat anti-Rabbit IgG DyLight™ 488 Conjugated Antibody
Goat
IgG (H&L)
DyLight™ 488
Polyclonal
IgG
Target Details
Rabbit
Rabbit IgG whole molecule
Conjugated Anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.
Application Details
Dot Blot, IF, Multiplex
FC, IHC, WB
- View References
Anti-Rabbit IgG (H&L) DyLight 488 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Rabbit IgG (H&L) DyLight 488 Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Wang J et al. (2024). CD8+ T cell infiltration and proliferation in the brainstem during experimental cerebral malaria. CNS Neurosci Ther.
Applications
IF, Confocal Microscopy
Li S et al. (2022). Zika Virus Infection Downregulates Connexin 43, Disrupts the Cardiomyocyte Gap Junctions and Induces Heart Diseases in A129 Mice. J Virol.
Applications
IF, Confocal Microscopy; IHC, ICC, Histology
Sanchez MM et al (2022). Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies. Biomolecules.
Applications
IF, Confocal Microscopy
Chen YL et al. (2021). Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells. Sci Rep.
Applications
FC, FACS, FLOW
Jayanthi B et al. (2021). A platform for post-translational spatiotemporal control of cellular proteins. Synth Biol (Oxf).
Applications
IF, Confocal Microscopy
Sanchez MM et al. (2021). Generation of Self-assembled Vascularized Human Skin Equivalents. J Vis Exp.
Applications
IF, Confocal Microscopy
Tian et al. (2017). Effects of TAZ on human dental pulp stem cell proliferation and migration. Molecular Medicine Reports
Applications
WB, IB, PCA
Dufour et al. (2016). Direct Correlation between Motile Behavior and Protein Abundance in Single Cells. PLOS Computational Biology
Applications
WB, IB, PCA
Dufour YS et al. (2016). Direct correlation between motile behavior and protein abundance in single cells. PLoS Comput Biol.
Applications
WB, IB, PCA
Yang FY et al. (2012). Focused ultrasound and interleukin-4 receptor-targeted liposomal doxorubicin for enhanced targeted drug delivery and antitumor effect in glioblastoma multiforme. J Control Release.
Applications
IF, Confocal Microscopy
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
