Mouse IgM (mu chain) Antibody Texas Red™ Conjugated

Datasheet

Mouse IgM (mu chain) Antibody Texas Red™ Conjugated

Name: Mouse IgM (mu chain) Antibody Texas Red™ Conjugated
Brand: Rockland Immunochemicals
Price: 233.0 USD
Availability: InStock

Goat Polyclonal

Item No. 610-1907

Size: 2 mg

Physical State: Lyophilized

Applications: Dot Blot

Reactivity: Mouse

Host Species: Goat

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Specifications for Mouse IgM (mu chain) Antibody Texas Red™ Conjugated

Product Details

Description: Goat Anti-Mouse IgM (mu chain) Antibody Texas Red™ Conjugated - 610-1907

Synonyms: Goat Anti-Mouse IgM (mu chain) Antibody Texas Red™ Conjugated, Goat Anti Mouse IgM mu chain Texas Red™ Conjugated Antibody

Host Species: Goat

Specificity: IgM µ chain

Conjugate: Texas Red®

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Mouse

Immunogen: Mouse IgM whole molecule

Purity/Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any un-wanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against other mouse heavy or light chain proteins.

Application Details

Tested Applications: Dot Blot

Application Note: Anti-Mouse IgM antibody Texas Red Conjugated has been tested by Dot Blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Formulation

Concentration: 2.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Anti-Mouse IgM antibody specifically detects mouse IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM Texas Red antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

Related Protocols

Adherent Cell Lysis Protocol

General Immunoblotting Protocol

Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.