Mouse IgG3 Antibody Peroxidase Conjugated Pre-adsorbed
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Datasheet

Mouse IgG3 Antibody Peroxidase Conjugated Pre-adsorbed

Goat Polyclonal

5 References
610-103-043
1 mg
Lyophilized
WB, ELISA
Mouse
Goat
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Product Details

Goat Anti-Mouse IgG3 (Gamma 3 chain) Antibody Peroxidase Conjugated (Min X Bv, Hu, and Rb Serum Proteins) - 610-103-043
goat anti-MOUSE IgG3 Antibody peroxidase Conjugation, goat anti-MOUSE IgG3 HRP Conjugated Antibody
Goat
IgG3
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Mouse
Native Protein
Mouse IgG3 heavy chain
Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

Application Details

ELISA
WB  - View References
Anti-Mouse IgG3 peroxidase antibody has been tested by ELISA and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods and anti IgG2b antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats. HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Mouse IgG3 Peroxidase Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG3 comprises almost 10% of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
(). A chimeric protein-based vaccine elicits a strong IgG antibody response and confers partial protection against Shiga toxin-producing Escherichia coli in mice. Front Immunol.
Applications
EIA, ELISA
(). Non-replicating adenovirus based Mayaro virus vaccine elicits protective immune responses and cross protects against other alphaviruses. PLoS Negl Trop Dis.
Applications
E, EIA
(). Blocking fatty acid synthase inhibits tumor progression of human osteosarcoma by regulating the human epidermal growth factor receptor 2/phosphoinositide 3-kinase/protein kinase B signaling pathway in xenograft models. Experimental and Therapeutic Medicine
Applications
WB, IB, PCA
(). Transcription factor NF‑YA promotes a malignant phenotype by upregulating fatty acid synthase expression. Molecular Medicine Reports
Applications
WB, IB, PCA
(). Salmonella porins induce a sustained, lifelong specific bactericidal antibody memory response. Immunology.
Applications
E, EIA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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