Mouse IgG2b (Gamma 2b chain) Antibody Rhodamine Conjugated

Datasheet

Mouse IgG2b (Gamma 2b chain) Antibody Rhodamine Conjugated

Name: Mouse IgG2b (Gamma 2b chain) Antibody Rhodamine Conjugated
Brand: Rockland Immunochemicals
Price: 220.0 USD
Availability: InStock
Rating: 5 (1 reviews)

Rabbit Polyclonal

1 References

Item No. 610-4042

Size: 1 mg

Physical State: Lyophilized

Applications: IF, Dot Blot

Reactivity: Mouse

Host Species: Rabbit

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Specifications for Mouse IgG2b (Gamma 2b chain) Antibody Rhodamine Conjugated

Product Details

Description: Rabbit Anti-Mouse IgG2b (Gamma 2b chain) Antibody Rhodamine Conjugated - 610-4042

Synonyms: Rabbit Anti-Mouse IgG2b (gamma 2b chain) Antibody rhodamine Conjugated, Rabbit Anti-Mouse IgG2b Antibody TRITC Conjugation

Host Species: Rabbit

Specificity: IgG2b

Conjugate: Rhodamine (TRITC)

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Mouse

Immunogen: Mouse IgG2b heavy chain

Purity/Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.

Application Details

Tested Applications: Dot Blot

Suggested Applications: IF - View References

Application Note: Anti-Mouse IgG2b Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Formulation

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Anti-Mouse IgG2b Rhodamine Antibody generated in rabbit detects reactivity to Mouse IgG2b (Gamma 2b chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25% of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

References (1)

Salmikangas P et al. (2003). Myotilin, the limb-girdle muscular dystrophy 1A (LGMD1A) protein, cross-links actin filaments and controls sarcomere assembly. Hum Mol Genet.

Applications

IF, Confocal Microscopy

PubMed

Related Protocols

Adherent Cell Lysis Protocol

General Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.