Datasheet

Mouse IgG2a Antibody Pre-adsorbed

Name: Mouse IgG2a Antibody Pre-adsorbed
Brand: Rockland Immunochemicals
Price: 189.0 USD
Availability: InStock
Rating: 5 (1 reviews)

Goat Polyclonal

1 References

Item No. 610-101-041

Size: 1 mg

Physical State: Liquid (sterile filtered)

Applications: ELISA, Dot Blot

Reactivity: Mouse

Host Species: Goat

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Specifications for Mouse IgG2a Antibody Pre-adsorbed

Product Details

Description: Goat Anti-Mouse IgG2a (Gamma 2a chain) Antibody (Min X to Bv, Hu & Rb Serum Proteins) - 610-101-041

Synonyms: goat anti-MOUSE IgG2a Antibody Conjugation, goat anti-Mouse IgG2a (gamma 2a) Conjugated Antibody

Host Species: Goat

Specificity: IgG2a

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Mouse

Immunogen Type: Native Protein

Immunogen: Mouse IgG2a heavy chain

Purity/Specificity: MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG2a. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG1, IgG2b and IgG3 reactivity was less than 5% against target (IgG2a, IgG). Cross-reactivity against Bovine is assessed only by IEP.

Application Details

Tested Applications: Dot Blot, ELISA

Application Note: Anti-Mouse IgG2a secondary antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG2a antibody based assays. Mouse IgG2a secondary antibody is available in a variety of formats.

Formulation

Concentration: 1.1 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Anti-Mouse IgG2a Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25% of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

References (1)

Seifert L et al. (2023). The classical pathway triggers pathogenic complement activation in membranous nephropathy. Nat Commun.

Applications

EIA, ELISA; IF, Confocal Microscopy

PubMed

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

General Immunoblotting Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.