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Mouse IgG2a Antibody Pre-adsorbed
Goat Polyclonal
1 References
610-101-041
1 mg
Liquid (sterile filtered)
ELISA, Dot Blot
Mouse
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Goat Anti-Mouse IgG2a (Gamma 2a chain) Antibody (Min X to Bv, Hu & Rb Serum Proteins) - 610-101-041
goat anti-MOUSE IgG2a Antibody Conjugation, goat anti-Mouse IgG2a (gamma 2a) Conjugated Antibody
Goat
IgG2a
Polyclonal
IgG
Target Details
Mouse
Native Protein
Mouse IgG2a heavy chain
MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG2a. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG1, IgG2b and IgG3 reactivity was less than 5% against target (IgG2a, IgG). Cross-reactivity against Bovine is assessed only by IEP.
Application Details
Dot Blot, ELISA
Anti-Mouse IgG2a secondary antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG2a antibody based assays. Mouse IgG2a secondary antibody is available in a variety of formats.
Formulation
1.1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
Anti-Mouse IgG2a Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25% of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Seifert L et al. (2023). The classical pathway triggers pathogenic complement activation in membranous nephropathy. Nat Commun.
Applications
EIA, ELISA; IF, Confocal Microscopy
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
