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Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed
Goat Polyclonal
4 References
610-1219
2 mg
Lyophilized
WB, IHC, IF
Mouse
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Goat Anti-Mouse IgG (H&L) Antibody Fluorescein Conjugated (Min X Human Serum Proteins) - 610-1219
Goat Anti-Mouse IgG Secondary Antibody fluorescein Conjugated, Goat Anti-Mouse IgG Antibody FITC Conjugated, GAM-FITC, Anti-mouse IgG secondary antibody, anti-mouse IgG Fluorescein conjugated secondary antibody
Goat
IgG (H&L)
Fluorescein (FITC)
Polyclonal
IgG
Target Details
Mouse
Mouse IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
Application Details
WB
IF, IHC
- View References
Mouse secondary antibody conjugated to FITC is available in a variety of formats. Anti-Mouse IgG Fluorescein Antibody has been tested by Western blot and is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti mouse IgG FITC is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Mouse IgG Fluorescein Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Saferali A, Tang AC, Strug LJ, et al. (2020). Immunomodulatory function of the cystic fibrosis modifier gene BPIFA1. PLoS One.
Applications
WB, IB, PCA
Vitacolonna M et al. (2017). In-vivo quantification of the revascularization of a human acellular dermis seeded with EPCs and MSCs in co-culture with fibroblasts and pericytes in the dorsal chamber model in pre-irradiated tissue. Cell Tissue Bank.
Applications
IF, Confocal Microscopy
Adefolaju GA et al. (2015). BAX/BCL-2 mRNA and protein expression in human breast MCF-7 cells exposed to drug vehicles-methanol and dimethyl sulfoxide (DMSO) for 24 hrs. Niger Med J.
Applications
IHC, ICC, Histology
Cavaglia M et al. (2001). Regional variation in brain capillary density and vascular response to ischemia. Brain Res.
Applications
IHC, ICC, Histology
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
