Mouse IgG (H&L) Antibody Peroxidase Conjugated
Donkey Polyclonal
8 References
610-703-002
2 mg
Lyophilized
WB, ELISA, IF
Mouse
Donkey
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Donkey Anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated - 610-703-002
Donkey Anti-Mouse IgG Antibody Peroxidase Conjugation, Donkey Anti-Mouse IgG HRP Conjugated Antibody
Donkey
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Mouse
Native Protein
Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in donkey
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Donkey Serum, Mouse IgG and Mouse Serum.
Application Details
ELISA, WB
IF
- View References
Donkey Anti-Mouse IgG HRP Conjugated Antibody has been tested by ELISA and western blot. This antibody is against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Formulation
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically mouse IgG (H&L). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Mouse peroxidase conjugated secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Chaudhari S et al. (2022). Renal TLR-7/TNF-α pathway as a potential female-specific mechanism in the pathogenesis of autoimmune-induced hypertension. Am J Physiol Heart Circ Physiol.
Applications
WB, IB, PCA
Hossain D et al. (2020). Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia. Cell Mol Life Sci.
Applications
WB, IB, PCA
Hossain D et al. (2020). Cep44 functions in centrosome cohesion by stabilizing rootletin. J Cell Sci.
Applications
WB, IB, PCA
Romero-Rodriguez A et al. (2020). The Clostridioides difficile Cysteine-Rich Exosporium Morphogenetic Protein, CdeC, Exhibits Self-Assembly Properties That Lead to Organized Inclusion Bodies in Escherichia coli. mSphere.
Applications
WB, IB, PCA
Lu X et al. (2019). 17β‑hydroxysteroid dehydrogenase 4 induces liver cancer proliferation‑associated genes via STAT3 activation. Oncol Rep.
Applications
WB, IB, PCA
Toots et al. (2018). Preventive treatment with liraglutide protects against development of glucose intolerance in a rat model of Wolfram syndrome. Scientific Reports
Applications
IF, Confocal Microscopy
Hossain D et al. (2018). HIV-1 Vpr hijacks EDD-DYRK2-DDB1DCAF1 to disrupt centrosome homeostasis. J Biol Chem.
Applications
WB, IB, PCA
Cruz JM, Hupper N, Wilson LS, et al. (2018). Protein kinase A activation inhibits DUX4 gene expression in myotubes from patients with facioscapulohumeral muscular dystrophy. J Biol Chem.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
