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Mouse IgG (H&L) Antibody Dylight™ 680 Conjugated
Goat Polyclonal
30 References
610-144-002
610-144-002-0.5
100 µg
5 x 100 µg
Lyophilized
Lyophilized
WB, IHC, IF, Dot Blot, IP
Mouse
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Goat Anti-Mouse IgG (H&L) Antibody DyLight™ 680 Conjugated - 610-144-002
Goat Anti-Mouse IgG Secondary Antibody DyLight™680 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™680 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™680 conjugated secondary antibody
Goat
IgG (H&L)
DyLight™ 680
Polyclonal
IgG
Target Details
Mouse
Mouse IgG, whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.
Application Details
Dot Blot, WB
IF, IHC, IP
- View References
Anti-Mouse IgG DyLight 680 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Mouse IgG DyLight 680 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Li L et al. (2024). Microglia Autophagy Mediated by TMEM166 Promotes Ischemic Stroke Secondary to Carotid Artery Stenosis. Aging Dis.
Applications
IF, Confocal Microscopy
Späte E et al. (2024). Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons. Glia.
Applications
WB, IB, PCA
Zhang Y et al. (2023). Testosterone reduces hippocampal synaptic damage in an androgen receptor-independent manner. J Endocrinol.
Applications
WB, IB, PCA
Wang, X et al. (2022). RNA-based therapies in animal models of Leber congenital amaurosis causing blindness. Precision Clinical Medicine
Applications
WB, IB, PCA
Zhang W, Zhang H, Yao W, et al. (2020). Morphometric, Hemodynamic, and Multi-Omics Analyses in Heart Failure Rats with Preserved Ejection Fraction. Int J Mol Sci.
Applications
WB, IB, PCA
Mitra S, Bodor DL, David AF, et al. (2020). Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin. Nat Commun.
Applications
WB, IB, PCA
Plana-Bonamaiso A et al. (2020). GCAP neuronal calcium sensor proteins mediate photoreceptor cell death in the rd3 mouse model of LCA12 congenital blindness by involving endoplasmic reticulum stress. Cell Death Dis.
Applications
WB, IB, PCA
Li R et al. (2020). RNF115 deletion inhibits autophagosome maturation and growth of gastric cancer Cell Death Dis.
Applications
WB, IB, PCA
Trevisiol A et al. (2020). Structural myelin defects are associated with low axonal ATP levels but rapid recovery from energy deprivation in a mouse model of spastic paraplegia. PLoS Biol.
Applications
WB, IB, PCA
Bacman SR et al. (2020). Manipulation of mitochondrial genes and mtDNA heteroplasmy. Methods Cell Biol.
Applications
WB, IB, PCA
Hong D et al. (2019). Deletion of TMEM268 inhibits growth of gastric cancer cells by downregulating the ITGB4 signaling pathway. Cell Death Differ.
Applications
WB, IB, PCA
Quancard J et al. (2019). An allosteric MALT1 inhibitor is a molecular corrector rescuing function in an immunodeficient patient. Nat Chem Biol.
Applications
WB, IB, PCA
Li B et al. (2019). Isobavachalcone exerts anti‑proliferative and pro‑apoptotic effects on human liver cancer cells by targeting the ERKs/RSK2 signaling pathway. Oncol Rep.
Applications
WB, IB, PCA
Sriramachanfran AM et al. (2019). Arkadia/RNF111 is a SUMO-targeted ubiquitin ligase with preference for substrates marked with SUMO1-capped SUMO2/3 chain. Nat Commun.
Applications
WB, IB, PCA
Bailetti AA et al. (2019). Enhancer of Polycomb and the Tip60 complex repress hematological tumor initiation by negatively regulating JAK/STAT pathway activity. Dis Model Mech.
Applications
WB, IB, PCA
Subramanian J et al. (2019). CPG15/neuritin mimics experience in selecting excitatory synapses for stabilization by facilitating PSD95 recruitment. Cell Rep.
Applications
WB, IB, PCA
Viringipurampeer IA et al. (2019). Cell death pathways in mutant rhodopsin rat models identifies genotype-specific targets controlling retinal degeneration. Mol Neurobiol.
Applications
WB, IB, PCA
Campbell LA et al. (2019). Gesicle-mediated delivery of CRISPR/Cas9 ribonucleoprotein complex for inactivating the HIV provirus. Mol Ther.
Applications
WB, IB, PCA
Zhang Y et al. (2019). Non-genomic mechanisms mediate androgen-induced PSD95 expression. Aging (Albany NY).
Applications
WB, IB, PCA
Lin et al. (2018). Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy. Cell Death & Disease
Applications
IHC, ICC, Histology; WB, IB, PCA
Pereira et al. (2018). mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial DNA levels. EMBO Molecular Medicine
Applications
WB, IB, PCA
Bacman SR et al. (2018). MitoTALEN reduces mutant mtDNA load and restores tRNA Ala levels in a mouse model of heteroplasmic mtDNA mutation. Nat Med.
Applications
WB, IB, PCA
Bacman, SR et al. (2018). MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse model of heteroplasmic mtDNA mutation. Nature Medicine
Applications
WB, IB, PCA
Li et al. (2017). Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality. Cell Death & Disease
Applications
WB, IB, PCA
Wang et al. (2017). Efficacy of Postnatal In Vivo Nonsense Suppression Therapy in a Pax6 Mouse Model of Aniridia. Molecular Therapy - Nucleic Acids
Applications
WB, IB, PCA
Xia et al. (2017). Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress. Cell Death & Disease
Applications
WB, IB, PCA
Shen et al. (2016). EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy. Cell Death & Disease
Applications
WB, IB, PCA
Xia D et al. (2016). MARCH2 regulates autophagy by promoting CFTR ubiquitination and degradation and PIK3CA-AKT-MTOR signaling. Autophagy.
Applications
WB, IB, PCA
Wang Z et al. (2014). PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1. Autophagy.
Applications
IP, Co-IP
Li Y et al. (2013). A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy. Autophagy.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
