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Mouse IgG (gamma chain) Antibody Peroxidase Conjugated
Rabbit Polyclonal
6 References
610-4312
1 mg
Lyophilized
WB, ELISA, Other
Mouse
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Rabbit Anti-Mouse IgG (gamma chain) Antibody Peroxidase Conjugated - 610-4312
rabbit anti-Mouse IgG Antibody peroxidase conjugation, rabbit anti-Mouse IgG gamma HRP conjugated Antibody
Rabbit
IgG (gamma chain)
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Mouse
Mouse IgG gamma heavy chain
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes.
Application Details
ELISA
Other, WB
- View References
Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is designed for ELISA, western blotting, and Immunohistochemistry. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Mouse IgG Peroxidase Antibody generated in rabbit detects reactivity to Mouse IgG gamma chain. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG gamma chain of the antibody is the F(c) region. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Poppenborg SM et al. (2016). Impact of anti-PEG IgM antibodies on the pharmacokinetics of pegylated asparaginase preparations in mice. Eur J Pharm Sci.
Applications
EIA, ELISA
Platt TL et al. (2016). Obesity, diabetes, and leptin resistance promote tau pathology in a mouse model of disease. Neuroscience
Applications
WB, IB, PCA
Beckett TL et al. (2012). Postmortem Pittsburgh Compound B (PiB) binding increases with Alzheimer's disease progression. Journal of Alzheimer's Disease : Jad
Applications
WB, IB, PCA
O'shannessy, DJ et al. (2011). Characterization of the human folate receptor alpha via novel antibody-based probes. Oncotarget
Applications
EIA, ELISA
McCall LI et al. (2010). Localization and induction of the A2 virulence factor in Leishmania: evidence that A2 is a stress response protein. Mol Microbiol.
Applications
WB, IB, PCA
Beckett TL et al. (2010). Effects of nonsteroidal anti-inflammatory drugs on amyloid-beta pathology in mouse skeletal muscle. Neurobiology of Disease
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
