Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody

Datasheet

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody

Name: Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody
Brand: Rockland Immunochemicals
Price: 189.0 USD
Availability: InStock

Rabbit Polyclonal

Item No. 610-401-C46

Size: 1 mg

Physical State: Liquid (sterile filtered)

Applications: ELISA

Reactivity: Mouse

Host Species: Rabbit

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Specifications for Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody

Product Details

Description: Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody - 610-401-C46

Synonyms: rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody, rabbit anti-Mouse IgG Antibody

Host Species: Rabbit

Specificity: IgG (gamma 1, 2a, 2b and 3 chain)

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Mouse

Immunogen Type: Native Protein

Immunogen: highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins

Purity/Specificity: Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.

Application Details

Tested Applications: ELISA

Application Note: Anti-Mouse IgG subclass pan reactive Secondary Antibody has been tested by ELISA and is designed for ELISA, Immunohistochemistry, western blotting as well as other anti-IgG antibody based assays. Mouse IgG secondary antibody is available in a variety of formats.

Formulation

Concentration: 1.0 mg/ml by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Anti-Mouse IgG Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG1, IgG2a, IgG2b and IgG3 chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Related Protocols

ELISA Protocol

Sandwich ELISA Protocol for Collagen

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.