Mouse IgG Fc Antibody DyLight™ 549 Conjugated

Anti-Tubulin and Anti-TNFa Antibodies - Western Blot

Properties of DyLight™ Fluorescent Dyes.

Datasheet

Mouse IgG Fc Antibody DyLight™ 549 Conjugated

Name: Mouse IgG Fc Antibody DyLight™ 549 Conjugated
Brand: Rockland Immunochemicals
Price: 111.0 USD
Availability: InStock

Goat Polyclonal

Item No. 610-142-003

Size: 100 µg

Physical State: Lyophilized

Reactivity: Mouse

Host Species: Goat

$111.00 /Per Item

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Specifications for Mouse IgG Fc Antibody DyLight™ 549 Conjugated

Product Details

Description: Goat Anti-Mouse IgG Fc Antibody DyLight™ 549 Conjugated - 610-142-003

Synonyms: Goat Anti Mouse IgG F(c) Antibody DyLight™ 549 Conjugated, Goat Anti-Mouse IgG Fc Antibody DyLight™ 549 Conjugated, Goat Anti Mouse IgG Fc Fragment Antibody DyLight™ 549 Conjugated

Host Species: Goat

Specificity: IgG Fc

Conjugate: DyLight™ 549

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Mouse

Immunogen: Mouse IgG F(c) fragment

Purity/Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab) This antibody will react with heavy chains of Mouse IgG. Minimal reactivity is expected against other Mouse immunoglobulins.

Application Details

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Formulation

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.