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Datasheet

Horse IgG (H&L) Antibody Peroxidase Conjugated

Rabbit Polyclonal

608-4302
2 mg
Lyophilized
ELISA
Horse
Rabbit
$189.00 /Per Item
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Rabbit Anti-Horse IgG (H&L) Antibody Peroxidase Conjugated - 608-4302
rabbit Anti-Horse IgG peroxidase Conjugated Antibody, rabbit Anti-Horse IgG Antibody peroxidase conjugate, rabbit Anti-Horse IgG Antibody HRP Conjugation
Rabbit
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Horse
Native Protein
Anti-Horse IgG whole molecule was produced by repeated immunization with Horse IgG whole molecule in rabbit.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG and Horse Serum.

Application Details

ELISA
Anti-Horse IgG Peroxidase Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation

2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
Anti-Horse IgG Peroxidase Antibody generated in rabbit detects horse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.