Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed
Donkey Polyclonal
7 References
605-703-125
1 mg
Lyophilized
WB, ELISA
Goat
Donkey
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Donkey Anti-Goat IgG (H&L) Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) - 605-703-125
Donkey anti-Goat IgG Antibody Peroxidase conjugation, Donkey anti-Goat IgG HRP conjugated antibody
Donkey
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Goat
Native Protein
Anti-Goat IgG (H&L) was produced by repeated immunization with Goat IgG whole molecule in donkey.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
Application Details
ELISA
WB
- View References
Anti-Goat IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Goat IgG Peroxidase antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Monette A. et al. (2020). Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking. Cell Rep.
Applications
WB, IB, PCA
Oud et al. (2017). Mutations in EXTL3 Cause Neuro-immuno-skeletal Dysplasia Syndrome. American Journal of Human Genetics
Applications
WB, IB, PCA
Ellison et al. (2017). IFN-γ alters the expression of diverse immunity related genes in a cell culture model designed to represent maturing neutrophils. PLOS One
Applications
WB, IB, PCA
Opperman CM et al. (2015). Tumor necrosis factor alpha stimulates p62 accumulation and enhances proteasome activity independently of ROS. Cell Biol Toxicol.
Applications
WB, IB, PCA
Tarassishin L et al. (2014). Interleukin-1-induced changes in the glioblastoma secretome suggest its role in tumor progression. Journal of Proteomics
Applications
WB, IB, PCA
Sishi BJN et al. (2013). Doxorubicin induces protein ubiquitination and inhibits proteasome activity during cardiotoxicity. Toxicology.
Applications
WB, IB, PCA
Miller DS et al. (2011). Preclinical efficacy studies of influenza A haemagglutinin precursor cleavage loop peptides as a potential vaccine. J Gen Virol.
Applications
E, EIA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
