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Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
Rabbit Polyclonal
6 References
605-4313-0100
605-4313
100 µg
1 mg
Lyophilized
Lyophilized
WB, ELISA
Goat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Rabbit Anti-Goat IgG (H&L) Antibody Peroxidase Conjugated (Min X Human Serum Proteins) - 605-4313
Goat IgG (H&L) Antibody, Rb-a-Goat IgG (H&L) Peroxidase conjugated, Goat IgG (H&L) Peroxidase Antibody in rabbit, rabbit anti-goat IgG peroxidase conjugated Secondary Antibody
Rabbit
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Goat
Native Protein
Goat IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Application Details
ELISA, WB
Anti-Goat IgG (H&L) peroxidase conjugated has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Goat IgG Peroxidase Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Ercan et al. (2017). Neuronal CTGF/CCN2 negatively regulates myelination in a mouse model of tuberous sclerosis complex. Journal of Experimental Medicine
Applications
WB, IB, PCA
Ko et al. (2017). Advanced glycation end products influence oral cancer cell survival via Bcl-xl and Nrf-2 regulation in vitro. Oncology Letters
Applications
WB, IB, PCA
Suzuki T et al. (2017). Efficient antibody production in the methylotrophic yeast Ogataea minuta by overexpression of chaperones. J Biosci Bioeng.
Applications
WB, IB, PCA
Tarassishin L et al. (2014). Interleukin-1-induced changes in the glioblastoma secretome suggest its role in tumor progression. Journal of Proteomics
Applications
WB, IB, PCA
van der Hoogt CC et al. (2007). Fenofibrate increases HDL-cholesterol by reducing cholesteryl ester transfer protein expression. J Lipid Res.
Applications
E, EIA
de Haan W et al. (2007). Atorvastatin increases HDL cholesterol by reducing CETP expression in cholesterol-fed APOE* 3-Leiden. CETP mice. Atherosclerosis.
Applications
E, EIA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
