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Goat IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed
Donkey Polyclonal
7 References
605-741-125
100 µg
Lyophilized
IF, Dot Blot, Multiplex
Goat
Donkey
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Donkey Anti-Goat IgG (H&L) Antibody DyLight™ 488 Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) - 605-741-125
donkey anti-Goat IgG Antibody DyLight™ 488 Conjugation, donkey anti-Goat IgG DyLight™ 488 Conjugated Antibody
Donkey
IgG (H&L)
DyLight™ 488
Polyclonal
IgG
Target Details
Goat
Goat IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
Application Details
Dot Blot
IF, Multiplex
- View References
Anti-Goat IgG DyLight 488 Antibody has been tested by dot blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Goat IgG DyLight Antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Onoda A et al. (2022). A Novel Staining Method for Detection of Brain Perivascular Injuries Induced by Nanoparticle: Periodic Acid-Schiff and Immunohistochemical Double-Staining. Front Toxicol.
Applications
IF, Confocal Microscopy
Iguchi, N et al. (2021). Functional constipation induces bladder overactivity associated with upregulations of Htr2 and Trpv2 pathways. Scientific Reports
Applications
IF, Confocal Microscopy
Onoda A, Kawasaki T, Tsukiyama K, Takeda K, Umezawa M. (2020). Carbon nanoparticles induce endoplasmic reticulum stress around blood vessels with accumulation of misfolded proteins in the developing brain of offspring. Sci Rep.
Applications
IF, Confocal Microscopy; Multiplex Assay
Umezawa M et al. (2018). Maternal inhalation of carbon black nanoparticles induces neurodevelopmental changes in mouse offspring. Particle and Fibre Toxicology
Applications
IF, Confocal Microscopy
Onoda et al. (2017). Dose-dependent induction of astrocyte activation and reactive astrogliosis in mouse brain following maternal exposure to carbon black nanoparticle. Particle and Fibre Toxicology
Applications
IF, Confocal Microscopy; Multiplex Assay
Onoda et al. (2017). Perivascular Accumulation of β-Sheet-Rich Proteins in Offspring Brain following Maternal Exposure to Carbon Black Nanoparticles. Frontiers in Cellular Neuroscience
Applications
IF, Confocal Microscopy; Multiplex Assay
Nishino T et al. (2012). Distinct distribution of the tensin family in the mouse kidney and small intestine. Exp Anim.
Applications
IF, Confocal Microscopy
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
