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Bovine IgG (H&L) Antibody Peroxidase Conjugated
Rabbit Polyclonal
201-4302
20 mg
Lyophilized
Bovine
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Rabbit Anti-Bovine IgG (H&L) Antibody Peroxidase Conjugated - 201-4302
Rabbit Anti-Bovine IgG HRP conjugation, rabbit Anti-Bovine peroxidase Conjugation
Rabbit
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Bovine
Bovine IgG whole molecule
Anti-BOVINE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
Application Details
Anti-BOVINE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:17,000 to 1:70,000 of the reconstitution concentration is suggested for this product.
Formulation
10.0 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Anti-BOVINE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated generated in goat detects specifically bovine IgG whole molecule. Anti-Bovine IgG antibody is ideal for investigators involved in Serum Protein Component Protein research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
