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Datasheet

UBE2D1 (UBCH5A) protein-HIS Epitope

009-001-U30S 009-001-U30
20 µg 50 µg
Liquid (sterile filtered) Liquid (sterile filtered)
H. sapiens (Human)
Human
E. coli
This product is discontinued

Product Details

UBE2D1 (UBCH5A) recombinant protein-HIS Epitope - 009-001-U30
E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A, Ubiquitin-conjugating enzyme E2 D1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1
Human
E. coli
Recombinant Protein

Target Details

UBE2D1
H. sapiens (Human)
Recombinant full length human UBE2D1 (UBCH5A) was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
?term=NM_003338 - Gene ID

Application Details

UBE2D1 Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. UBE2D1 Protein is suitable for use in Western Blot. Expect a band approximately ~17kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

0.1 µg/µL
See application note.

Shipping & Handling

Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.
UBE2D1 (UBCH5A) or ubiquitin-conjugating enzyme E2D1 is a member of the E2 ubiquitin-conjugating enzyme family which is closely related to a stimulator of iron transport (SFT). UBE2D1 is significantly upregulated in livers of iron-overloaded patients with hereditary hemochromatosis (1). UBE2D1 also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF-1a by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases (2). UBE2D1 Protein is ideal for investigators involved in Signaling Proteins Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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