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Surface Lipoprotein p27 Control Protein
1 References
000-001-C30
100 µg
Liquid (sterile filtered)
WB, SDS-PAGE, Biochemical Assay
Borrelia burgdorferi
E. coli
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Surface Lipoprotein p27 Control Protein - 000-001-C30
BBA060 protein, Borrelia burgdorferi p27, Surface lipoprotein p27, control protein
Borrelia burgdorferi
E. coli
Recombinant Protein
Target Details
BB_A60 - View All BB_A60 Products
Surface Lipoprotein p27 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Surface Lipoprotein p27 and was estimated to be greater than 90% pure.
Application Details
SDS-PAGE, WB
Biochemical Assay
- View References
Surface Lipoprotein p27 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 73.3 kDa for p27-MBP, (30.9 kDa for p27 and 42.4 kDa for MBP) in size corresponding to Surface Lipoprotein p27 by Western blotting in the appropriate cell lysate or extract. Surface Lipoprotein p27 protein was tested in SDS-page and western blot.
Formulation
1.0 mg/mL by modified Lowry assay
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Expiration date is six (6) months from date of receipt.
Surface Lipoprotein p27 of Borrelia burgdorferi is a surface-exposed lipoprotein that has been shown (by Western blot and Northern blot) to be expressed in the European B. burgdorferi strain B29, but not in the American strain B31. Cell envelope proteins of bacterial pathogens play important roles in the host-parasite interactions that occur during infection, including cell adherence, cell invasion, and immune cell activation or evasion. p27 is a basic protein of 248 amino acids with a typical prokaryotic leader sequence of 17 amino acid residues at the N-terminus of the proposed translation product. The p27 gene is located on a linear plasmid of a size of approximately 55 kb. Borrelia spirochetes are unique among diderm bacteria in their abundance of surface-displayed lipoproteins, some of which play important roles in the pathogenesis of Lyme disease and relapsing fever. There is evidence that Borrelia lipoproteins are specifically targeted to the bacterial surface, but that they can be retained in the periplasm by sequence-specific signals. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.
Bowman KA. et al. (2024). Borrelia-specific antibody profiles and complement deposition in joint fluid distinguish antibiotic-refractory from -responsive Lyme arthritis. iScience.
Applications
Protein Assay
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
