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Sheep IgG Fab
3 References
013-0105
2 mg
Liquid (sterile filtered)
IF, LFA, Other
Sheep
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Sheep IgG Fab Fragment - 013-0105
Sheep IgG Fab fragment
Sheep
IgG Fab
Native Protein
Target Details
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, anti-Sheep IgG and anti-Sheep IgG F(ab’)2. No reaction was observed against anti-Sheep IgG F(c) or anti- Papain.
Application Details
IF, Other, LFA
- View References
Sheep IgG Fab fragment reagents are ideal for ELISA, western blotting, lateral flow, Immunohistochemistry, as well as other antibody detection methods.
Formulation
2.0 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
Sheep IgG Fab Fragment - secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.
Bian L et al. (2021). Rapid monitoring of vancomycin concentration in serum using europium (Ⅲ) chelate nanoparticle-based lateral flow immunoassay. Front Chem.
Applications
Lateral Flow (LFA)
Gross, NB et al. (2019). Endogenous Na+, K+-ATPase inhibitors and CSF [Na+] contribute to migraine formation. PloS One
Applications
Immunization
Miura, Y. et al. (2001). Critical contribution of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) to apoptosis of human CD4+ T cells in HIV-1-infected hu-PBL-NOD-SCID mice. The Journal of Experimental Medicine
Applications
TUNEL
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
