IL-6 Protein Mouse Recombinant Protein - SDS-PAGE

Datasheet

rMouse IL-6 Protein

Name: rMouse IL-6 Protein
Brand: Rockland Immunochemicals
Price: 292.0 USD
Availability: OutOfStock
Rating: 5 (1 reviews)

1 References

Item No. 010-001-310

Size: 10 µg

Physical State: Lyophilized

Applications: WB, IHC, SDS-PAGE

Origin: Mouse

Expressed in: E. coli

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Specifications for rMouse IL-6 Protein

Product Details

Description: IL-6 Mouse Recombinant Protein - 010-001-310

Synonyms: Interleukin-6 cytokine, IL-6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, IFN-beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, Interleukin HP-1

Species of Origin: Mouse

Expressed in: E. coli

Type: Recombinant Protein

Low Endotoxin:

Target Details

Gene Name: Il6 - View All Il6 Products

Purity/Specificity: Purity greater than 95% as determined by reducing and non-reducing SDS-PAGE.

Database Links:

P08505 - UniProtKB

Application Details

Tested Applications: SDS-PAGE

Suggested Applications: IHC, WB - View References

Application Note: Interleukin-6 (IL-6) has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-6 (IL-6) in immunological assays. It is recommended to reconstitute the lyophilized recombinant M-IL-6 in 10 mM HCl to 0.1-1.0 mg/mL to regain full activity; this can then be further diluted to other aqueous solutions.

Formulation

Concentration: 0.1 mg/mL by UV absorbance at 280 nm

Buffer: 0.1% Trifluoroacetic acid

Preservative: None

Stabilizer: None

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiration: Expiration date is six (6) months from date of receipt.

Background

Interleukin (IL)-6, also know as BCDG, BCGF and BSF-2, is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Mouse IL-6 produced in E. coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21,709 Daltons.

References (1)

Turnis M. et al. (2020). Requirement for Anti-Apoptotic MCL-1 during Early Erythropoiesis. Blood

Applications

IHC, ICC, Histology; WB, IB, PCA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunohistochemistry (IHC) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.