SDS-PAGE of Human Interleukin-17AF Heterodimer Recombinant Protein

Datasheet

rHuman IL-17AF Heterodimer Protein

Name: rHuman IL-17AF Heterodimer Protein
Brand: Rockland Immunochemicals
Price: 292.0 USD
Availability: OutOfStock

Item No. 009-001-V56-0005

Size: 5 µg

Physical State: Lyophilized

Applications: SDS-PAGE, Cellular Assay

Origin: Human

Expressed in: E. coli

$121.00 /Per Item

5 µg $121.00

25 µg $292.00

100 µg $999.00

1 mg $8,235.00

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Specifications for rHuman IL-17AF Heterodimer Protein

Product Details

Description: Human Interleukin-17AF Heterodimer Recombinant Protein - 009-001-V56-0005

Synonyms: IL17 heterodimer, IL17AF heterodimer, CTLA-8 ML-1 dimer, Interleukin 17AF, Interleukin-17AF heterodimer

Species of Origin: Human

Expressed in: E. coli

Type: Recombinant Protein

Low Endotoxin:

Target Details

Gene Name: IL17A/IL17F - View All IL17A/IL17F Products

Purity/Specificity: Interleukin-17AF Heterodimer purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Database Links:

Q16552 - UniProtKB

Application Details

Tested Applications: SDS-PAGE

Suggested Applications: Cellular Assay - View References

Application Note: Interleukin-17AF Heterodimer Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-17AF Heterodimer in immunological assays.

Formulation

Buffer: 0.1% Trifluoroacetic acid

Preservative: None

Stabilizer: None

Reconstitution Volume: 5µl (5-50µl)

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiration: Expiration date is six (6) months from date of receipt.

Background

Interleukin-17AF (IL-17AF) is a member of the IL-17 family of proteins produced by a subset of T cells, called Th17, following stimulation with IL-23. Since IL-17AF is thought to signal through the IL-17RA receptor, its biological function is similar to that of IL-17A in that it induces the production of a variety of chemokines, in addition to airway neutrophilia. In regard to these functions, IL-17AF has less activity than the IL-17A homodimer but, greater activity than the IL-17F homodimer. Human and rat IL-17AF both show activity on mouse cells. Recombinant human IL-17AF is a non-glycosylated heterodimer, containing one IL-17A subunit and one IL-17F subunit. The dimer has a total of 271 amino acids, with an approximate molecular weight of 30.7 kDa.

Related Protocols

Azide Removal by Dialysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.