SDS-PAGE of Human Growth and Differentiation Factor-15 (D variant) Recombinant Protein

Datasheet

rHuman GDF15 (D variant) Protein

Name: rHuman GDF15 (D variant) Protein
Brand: Rockland Immunochemicals
Price: 292.0 USD
Availability: OutOfStock

Item No. 009-001-V09-0005

Size: 5 µg

Physical State: Lyophilized

Applications: SDS-PAGE

Origin: Human

Expressed in: E. coli

$121.00 /Per Item

5 µg $121.00

20 µg $292.00

100 µg $735.00

1 mg $6,045.00

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Specifications for rHuman GDF15 (D variant) Protein

Product Details

Description: Human Growth and Differentiation Factor-15 (D variant) Recombinant Protein - 009-001-V09-0005

Synonyms: Macrophage inhibitory cytokine 1 (MIC-1), NSAID-activated gene 1 protein (NAG-1), NSAID-regulated gene 1 protein (NRG-1), Placental TGFβ, Placental bone morphogenetic protein, Prostate differentiation factor

Species of Origin: Human

Expressed in: E. coli

Type: Recombinant Protein

Low Endotoxin:

Target Details

Gene Name: GDF15 - View All GDF15 Products

Purity/Specificity: Growth and Differentiation Factor-15 (D variant) purity was determined to be greater than 95% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Database Links:

Q99988 - UniProtKB

Application Details

Tested Applications: SDS-PAGE

Application Note: Growth and Differentiation Factor-15 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Growth and Differentiation Factor-15 in immunological assays.

Formulation

Buffer: 0.1% Trifluoroacetic acid

Preservative: None

Stabilizer: None

Reconstitution Volume: 5µl (5-50µl)

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiration: Expiration date is six (6) months from date of receipt.

Background

Growth and Differentiation Factor 15 (GDF-15) is a TGFβ family member, made by the placenta and heart tissues, that has a role in regulating inflammatory and apoptotic pathways. GDF-15 has become an immerging marker of early heart disease and has the potential as being used as a molecule for screening patients for early heart failure. Recombinant human GDF-15 D is a non-glycosylated, disulfide linked homodimer, containing two identical 113 amino acid chains, with a total molecular weight of 24.5 kDa. There is a His to an Asp substitution at position 7.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.