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Datasheet

rHuman EGF Protein

009-F01-C26-1000
1 mg
Lyophilized
Cellular Assay
Human
E. coli
This product is discontinued

Product Details

Human Epidermal Growth Factor Recombinant Protein (Animal Free) - 009-F01-C26-1000
Urogastrone, URG
Human
E. coli
Recombinant Protein

Target Details

EGF  - View All EGF Products
Epidermal Growth Factor (EDF) is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 95% as determined by analysis by HPLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-PAGE.
P01133 - UniProtKB

Application Details

Cellular Assay  - View References
Epidermal Growth Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Epidermal Growth Factor in immunological assays.

Formulation

0.1 mg/mL by UV absorbance at 280 nm
0.1% Trifluoroacetic acid
None
None
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Expiration date is six (6) months from date of receipt.
Epidermal Growth Factor (EGF) is a growth factor that stimulates the proliferation of epithelial and epidermal cells. EGF family members are characterized by three intramolecular disulfide bonds and can bind to four different receptor tyrosine kinases known as EGFR/ErbB1, ErbB2, ErbB3, and ErbB4. EGF is overexpressed in multiple tumor cell lines and promotes resistance to chemotherapy and radiation treatments. Recombinant human EGF is a single, non-glycosylated protein containing 53 amino acids, with a molecular weight of 6.2 kDa.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.