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Protein G DyLight™ 680 Conjugated
PG00-44
100 µg
Lyophilized
Streptococcus sp.
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Protein G DyLight™ 680 Conjugated - PG00-44
ProG, Streptococcus G protein, Protein G DyLight™ 680 Conjugated
Streptococcus sp.
DyLight™ 680
Target Details
Protein G DyLight™680 is chromatographically pure recombinant Protein G and shows predominantly a single band by SDS-PAGE. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Protein G. No reaction was observed against anti-Protein A.
P19909 - UniProtKB
Application Details
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. While native Protein G binds to albumin, recombinant Protein G is designed to contain only immunoglobulin binding domains to ensure the maximum specific IgG binding capacity. Protein G is often immobilized on solid surface such as Sepharose™, latex or magnetic particles for IgG purification from ascites, serum or hybridoma culture media. Protein G is also coupled with reporter molecules such as a fluorescein, peroxidase, biotin, DyLight™ and other detection reagents.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
