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Mouse IgG Fab fragment
3 References
010-0105
2 mg
Liquid (sterile filtered)
IF, SDS-PAGE, Biochemical Assay, Multiplex
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Mouse IgG Fab Fragment - 010-0105
Mouse Immunoglobulin Fab, F(ab), Fragment antigen-binding
Mouse
IgG Fab
Native Protein
Target Details
Mouse IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Mouse IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG, anti-Mouse IgG F(ab’)2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti- Papain.
Application Details
SDS-PAGE
Biochemical Assay, IF, Multiplex
- View References
Mouse IgG Fab Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Formulation
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
Pleiner T et al. (2018). A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol.
Applications
Dot Blot Assay
Sasakura Y et al. (2004). Protein microarray system for detecting protein-protein interactions using an anti-His-tag antibody and fluorescence scanning: effects of the heme redox state on protein-protein interactions of heme-regulated phosphodiesterase from Escherichia coli. Anal Chem.
Applications
IF, Confocal Microscopy; Multiplex Assay
Rui H et al. (1994). JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endocrinology.
Applications
Cell Proliferation Assay
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
