SDS-PAGE of Mouse IgG Fab Fragment (p/n 010-0105)

Dot Blot results using Mouse Fab fragment

Datasheet

Mouse IgG Fab fragment

Name: Mouse IgG Fab fragment
Brand: Rockland Immunochemicals
Price: 219.0 USD
Availability: InStock
Rating: 5 (3 reviews)

3 References

Item No. 010-0105

Size: 2 mg

Physical State: Liquid (sterile filtered)

Applications: IF, SDS-PAGE, Biochemical Assay, Multiplex

Origin: Mouse

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Specifications for Mouse IgG Fab fragment

Product Details

Description: Mouse IgG Fab Fragment - 010-0105

Synonyms: Mouse Immunoglobulin Fab, F(ab), Fragment antigen-binding

Species of Origin: Mouse

Format: IgG Fab

Type: Native Protein

Target Details

Purity/Specificity: Mouse IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Mouse IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG, anti-Mouse IgG F(ab’)2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti- Papain.

Application Details

Tested Applications: SDS-PAGE

Suggested Applications: Biochemical Assay, IF, Multiplex - View References

Application Note: Mouse IgG Fab Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation

Concentration: 2.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.

References (3)

Pleiner T et al. (2018). A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol.

Applications

Dot Blot Assay

PubMed

Sasakura Y et al. (2004). Protein microarray system for detecting protein-protein interactions using an anti-His-tag antibody and fluorescence scanning: effects of the heme redox state on protein-protein interactions of heme-regulated phosphodiesterase from Escherichia coli. Anal Chem.

Applications

IF, Confocal Microscopy; Multiplex Assay

PubMed

Rui H et al. (1994). JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endocrinology.

Applications

Cell Proliferation Assay

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Biotin Binding Assay Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Nuclear & Cytoplasmic Extract Protocol

Peptide Competition Assay (PCA) Protocol

Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.