SDS PAGE Results of human Collagen Type II

Datasheet

Human Collagen Type II

Name: Human Collagen Type II
Brand: Rockland Immunochemicals
Price: 685.0 USD
Availability: InStock
Rating: 5 (1 reviews)

1 References

Item No. 009-001-104

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, SDS-PAGE

Origin: Human

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Specifications for Human Collagen Type II

Product Details

Description: Human Collagen Type II (ADULT KNEE CARTILAGE) - 009-001-104

Synonyms: Type II collagen, collagen II, human collagen, hyaline collagen

Species of Origin: Human

Type: Native Protein

Target Details

Gene Name: COL2A1 - View All COL2A1 Products

Purity/Specificity: Human Collagen Type II has been prepared from adult human knee cartilage and is chromatographically and immunologically pure. Human Collagen Type II is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Human Collagen Type II reacts with anti-Collagen Type II. Reaction with Rockland’s anti-Collagen I, III, IV, V or VI is negligible.

Database Links:

P02458 - UniProtKB

CAA34683.1 - NCBI Protein

1280 - Gene ID

Application Details

Tested Applications: SDS-PAGE

Suggested Applications: WB - View References

Application Note: Human Collagen Type II has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation

Concentration: 1.0 mg/mL by nanodrop at 205 nm

Buffer: 0.5 M Acetic Acid

Preservative: 0.01% (w/v) Sodium Azide

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is six (6) months from date of receipt.

Background

Human Collagen Type II is one of a family of proteins found particularly in the flesh and connective tissues of mammals (approximately one-third of the body's total protein). Over two dozen types of collagen have been described; Type II is found predominantly in hyaline cartilage (50% of the protein in hyaline is collagen type II) and is also found in the vitreous humor of the eye.

References (1)

Wang C et al. (2020). Type III collagen is a key regulator of the collagen fibrillar structure and biomechanics of articular cartilage and meniscus. Matrix Biol.

Applications

WB, IB, PCA

PubMed

Frequently Asked Questions (8)

What is the source of the collagen proteins?

Collagen proteins are purified from various sources including bovine or human placenta, skin, nasal tissue, and knee cartilage. The purity of the protein is analyzed by SDS-PAGE.

How are the collagen proteins purified?

The proteins were purified by a proprietary method that includes mechanical separation and salt precipitation. Due to the nature and close association of certain collagen proteins, it is difficult to fully separate each collagen.

In what format are the collagen proteins provided?

Collagen proteins are provided in liquid format at a concentration of around 1 mg/mL. The collagen proteins are solubilized, maintaining their native structure. No enzymatic treatment is performed. The low pH of the buffer is essential in maintaining the solubility of the collagen protein.

In what buffer are the collagen proteins provided?

0.5 M Acetic Acid, pH 2.5 with 0.01% (w/v) Sodium Azide.

What are the recommended SDS-PAGE conditions for collagen proteins?

The recommended buffer for SDS-PAGE is 2X SDS buffer with 8 M Urea. The low pH of the buffer maintains the solubility of the collagen proteins. Also, low-density gels such as 3-8% Tris Acetate should be used. Collagen proteins can be reduced with DTT.

What are the recommended storage conditions for collagen proteins?

Collagen proteins should always be stored at 4°C. It is important to never let the collagen proteins freeze because this will degrade the protein. Please be sure to maintain 4°C temperature during assays such as SDS-PAGE.

How was the concentration determined?

The concentration of the collagen proteins was measured by absorbance via nanodrop at 205 nm. When measuring concentration, please be sure to blank with the same buffer as the protein product (0.5 M Acetic Acid).

Are collagen proteins suitable for cell culture?

Since no testing for endotoxin or other contaminants was performed, we do not recommend the use of this product for cell culture.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.; This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.