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ErpN/OspE Control Protein
1 References
000-001-C10
100 µg
Liquid (sterile filtered)
WB, SDS-PAGE, Biochemical Assay
Borrelia burgdorferi
E. coli
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
ErpN/OspE Control Protein - 000-001-C10
ErpN, ErpA, outer surface protein E, Borrelia burgdorferi OspE, ospE/F-Related Protein N, control protein
Borrelia burgdorferi
E. coli
Recombinant Protein
Target Details
erpN, BB_L39 - View All erpN, BB_L39 Products
ErpN/OspE is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified ErpN/OspE and was estimated to be greater than 90% pure.
Application Details
SDS-PAGE, WB
Biochemical Assay
- View References
ErpN/OspE is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 59.5 kDa for OspE-MBP, (17.1 kDa for OspE and 42.4 for MBP) in size corresponding to ErpN/OspE by Western blotting in the appropriate cell lysate or extract. ErpN/OspE protein has been tested in SDS-page and western blot.
Formulation
1mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Expiration date is six (6) months from date of receipt.
ErpN (OspE/F-Related Protein N), is from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. Erp proteins from Borrelia burgdorferi are postulated to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, indicative of Erp synthesis during the initial stages of vertebrate infection. It is postulated that surface-exposed Erp proteins could facilitate interactions with host tissues during the establishment of vertebrate infection. Lyme disease proteins are ideal for researchers interested in immunology, neurology, and rheumatology, coinfections, autoimmune, and neurodegenerative diseases.
Bowman KA. et al. (2024). Borrelia-specific antibody profiles and complement deposition in joint fluid distinguish antibiotic-refractory from -responsive Lyme arthritis. iScience.
Applications
Protein Assay
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.