EGF Human Recombinant Epidermal Growth Factor
2 References
009-001-C26
500 µg
Lyophilized
Cellular Assay
Human
Shipping info:
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Product Details
EGF Human Recombinant Epidermal Growth Factor - 009-001-C26
Epidermal growth factor, Urogastrone
Human
Recombinant Protein
Target Details
EGF - View All EGF Products
Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE, against known standard.
P01133 - UniProtKB
Application Details
Cellular Assay
- View References
EGF Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-EGF in immunological assays.
Formulation
0.1 mg/mL by UV absorbance at 280 nm
0.1% Trifluoroacetic acid
None
None
5.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Wet Ice
Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Expiration date is six (6) months from date of receipt.
EGF (Epidermal Growth Factor) protein, of the superfamily with the same name, is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. It is a magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4 (HOMG4). Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants. This protein exists in the membrane as a single-pass type I membrane protein, and contains 9 EGF-like domains as well as 9 LDL-receptor class B repeats. It is significantly expressed in kidney, salivary gland, cerebrum and prostate.
Khan SA et al. (2021). Probing the signaling requirements for naive human pluripotency by high-throughput chemical screening. Cell Rep.
Applications
Cell Culture
Dong C et al. (2020). Derivation of trophoblast stem cells from naïve human pluripotent stem cells eLife.
Applications
Cell Culture
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
